Western blot analysis of Recombinant Mouse Lefty-1 Protein using PA5-19507, Lefty primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19507 staining of Recombinant Human Lefty-1 Protein at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19507 targets Lefty in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-19507 immunogen is synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty.
PA5-19507 detects Lefty which has a predicted molecular weight of approximately 44 kDa.
This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.