|ChIP assay (ChIP)||1.5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:50 - 1:200|
|Western Blot (WB)||1:200 - 1:1000|
|Western Blot (WB)||See 6 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Species reactivity||Human, Mouse, Sheep, Rat|
|Published species||Avian, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-051 detects leptin from human, mouse, sheep and rat samples.
PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.
The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.
Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD295 antigen; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; obese, mouse, homolog of; Obesity factor; obesity protein
Gene Aliases: LEP; LEPD; OB; obese; OBS
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