Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Leptin polyclonal antibody (Product # PA1-052) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse, Pig|
|Published species reactivity||Pig, Sheep, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-052 detects leptin from human, mouse, and porcine samples.
PA1-052 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~16 kDa protein representing leptin from human serum.
The PA1-052 immunogen is a synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin. This sequence is 94% conserved between human and bovine.
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.
Ob knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Expression of leptin and its long form receptor at the porcine maternal-fetal interface: contrasting healthy and arresting conceptus attachment sites during early and mid-pregnancy.
PA1-052 was used in immunohistochemistry - paraffin section and western blot to investigate the role of LEP and its long form receptor in porcine conceptus development
|Kerr A,Kridli RT,Khalaj K,Wessels JM,Hahnel A,Tayade C||Reproductive biology and endocrinology : RB and E (12:null)||2014|
The effect of leptin on luteal angiogenic factors during the luteal phase of the estrous cycle in goats.
PA1-052 was used in immunohistochemistry - paraffin section to measure FGF2, Ang1, VEGF, ObRb, and leptin expression in the corpus luteum of goats
|Wiles JR,Katchko RA,Benavides EA,O'Gorman CW,Escudero JM,Keisler DH,Stanko RL,Garcia MR||Animal reproduction science (148:121)||2014|
In vitro effects of luteinizing hormone, progesterone and oestradiol-17ß on leptin gene expression and leptin secretion by porcine luteal cells obtained in early pregnancy.
PA1-052 was used in immunocytochemistry to study the expression and secretion of leptin by early pregnancy pig luteal cells and the effects of luteinizing hormone, progesterone and oestradiol
|Siawrys G,Smolinska N||Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (64:513)||2013|
Direct in vitro effect of LH and steroids on leptin gene expression and leptin secretion by porcine luteal cells during the mid-luteal phase of the estrous cycle.
PA1-052 was used in immunocytochemistry to study leptin expression and secretion by pig luteal cells during the mid-luteal cycle and the effects of luteinizing hormone and steroid hormones
|Siawrys G,Smolinska N||Reproductive biology (12:317)||2012|
Human skeletal muscle releases leptin in vivo.
PA1-052 was used in western blot to study the release of leptin from human skeletal leg muscle in vivo
|Wolsk E,Mygind H,Grøndahl TS,Pedersen BK,van Hall G||Cytokine (60:667)||2012|
IL-6 regulates exercise and training-induced adaptations in subcutaneous adipose tissue in mice.
PA1-052 was used in western blot to investigate the role of IL-6 in exercise-induced adipose tissue changes
|Brandt C,Jakobsen AH,Adser H,Olesen J,Iversen N,Kristensen JM,Hojman P,Wojtaszewski JF,Hidalgo J,Pilegaard H||Acta physiologica (Oxford, England) (205:224)||2012|
Effects of acute fasting and age on leptin and peroxisome proliferator-activated receptor gamma production relative to fat depot in immature and mature pigs.
PA1-052 was used in western blot to investigate the effect of acute fasting on leptin and PPAR-gamma production in pigs
|O'Gorman CW,Stanko RL,Keisler DH,Garcia MR||Journal of animal physiology and animal nutrition (94:e266)||2010|
Expression of leptin and long-form leptin-receptor proteins in porcine hypothalamus during oestrous cycle and pregnancy.
PA1-052 was used in western blot to study hypothalamic expression of leptin and long-form leptin-receptor proteins during oestrous cycle and pregnancy
|Siawrys G,Kaminski T,Smolinska N,Przala J||Reproduction in domestic animals = Zuchthygiene (44:920)||2009|
Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action.
PA1-052 was used in western blot to investigate the relationship between leptin and body lipid content in mice
|Frederich RC,Hamann A,Anderson S,Löllmann B,Lowell BB,Flier JS||Nature medicine (1:1311)||1995|