PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues.
PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract.
The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure.
Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B219; CD295; CD295 antigen; HuB219; LEP-R; LEPR; LeprB; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R; OBR
Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR