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Invitrogen
Leptin Receptor Polyclonal Antibody
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Product Details
PA1-053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.
Conjugate
Form
Liquid
Concentration
10 mg/mL
Amount
1 mg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues.
PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract.
The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B219; CD antigen CD295; CD295; CD295 antigen; cytokine receptor; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R; splice variant form 12.1; Ob receptor; splice variant form 13.2; Ob receptor; splice variant form 6.4; Ob receptor; unnamed protein product
Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR
UniProt ID: (Human) P48357, (Rat) Q62959, (Mouse) P48356
Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847
angiogenesis
glycogen metabolic process
gluconeogenesis
energy reserve metabolic process
phagocytosis
cell surface receptor signaling pathway
multicellular organism development
cholesterol metabolic process
positive regulation of cell proliferation
negative regulation of autophagy
glial cell proliferation
cytokine-mediated signaling pathway
sexual reproduction
T cell differentiation
leptin-mediated signaling pathway
glucose homeostasis
response to leptin
negative regulation of gluconeogenesis
regulation of bone remodeling
system development
regulation of transport
regulation of feeding behavior
energy homeostasis
receptor signaling pathway via STAT
bone growth
positive regulation of cold-induced thermogenesis
transport across blood-brain barrier
ovulation from ovarian follicle
response to hypoxia
generation of precursor metabolites and energy
G-protein coupled receptor signaling pathway
female pregnancy
response to nutrient
response to nicotine
eating behavior
positive regulation of MAPK cascade
response to estrogen
negative regulation of hydrolase activity
cellular response to organic substance
signal transduction
regulation of metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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