Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Leptin Receptor Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
PA1-053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.
Conjugate
Form
Liquid
Concentration
10 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues.
PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract.
The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.
Target Information
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B219; CD antigen CD295; CD295; CD295 antigen; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R
Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR
UniProt ID: (Human) P48357, (Rat) Q62959, (Mouse) Q64309
Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847
angiogenesis
energy reserve metabolic process
cell surface receptor signaling pathway
multicellular organism development
cholesterol metabolic process
negative regulation of autophagy
sexual reproduction
T cell differentiation
leptin-mediated signaling pathway
glucose homeostasis
response to leptin
negative regulation of gluconeogenesis
regulation of bone remodeling
negative regulation of hydrolase activity
regulation of feeding behavior
energy homeostasis
bone growth
regulation of energy homeostasis
ovulation from ovarian follicle
response to hypoxia
generation of precursor metabolites and energy
G-protein coupled receptor signaling pathway
female pregnancy
response to nutrient
cytokine-mediated signaling pathway
response to nicotine
eating behavior
positive regulation of MAPK cascade
response to estrogen
cellular response to organic substance
signal transduction
regulation of metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support