Invitrogen

Leptin Receptor Polyclonal Antibody

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Leptin Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Leptin Receptor in paraffin embedded human liver using a Leptin Receptor polyclonal antibody (Product #PA5-18522) at a concentration of 4 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

Leptin Receptor Antibody in Western Blot (WB) — Western blot analysis of Leptin Receptor by a Leptin Receptor monoclonal antibody (Product # PA5-18522) at a concentration of 0.1 µg/mL. Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Leptin Receptor Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F)) — Fig. 4 Immunofluorescence staining of defect areas at 6 weeks after surgery. ( A ) Representative images of immunofluorescence staining of CGRP and LepR. ( B ) Representative images of CGRP and Emcn staining. ( C ) Representative images of CGRP and CD31 staining. ( D ) Quantification of CGRP, LepR, Emcn and CD31 median fluorescence intensity (MFI) per field view. Nuclei were stained with DAPI (blue). Scale bar: 100 mum. Data represent means +- standard deviations (n = 6). For all charts, groups labeled with different lowercase letters are significantly different (p < 0.05). Fig. 4

Leptin Receptor Antibody in Immunohistochemistry (IHC) — Fig. 7 Sensory nerve-derived Sema3A promotes osteogenesis and angiogenesis in SCS-mediated bone regeneration. ( A ) Micro-CT analysis of bone regeneration at 12 weeks. 3D reconstructed superficial and interior images of femoral condyle defects in different groups. Scale bar: 4 cm. ( B ) Quantitative analysis of bone volume/total volume (TV/BV), bone mineral density (BMD) and Trabecular Thickness (Tb.Th) (n = 6). ( C ) Representative images of immunofluorescence staining of Leptin Receptor and CGRP. ( D ) Representative images of immunofluorescence staining of CGRP and Emcn. ( E ) Representative images of immunofluorescence staining of CGRP and CD31. ( F ) Quantification of CGRP, LepR, Emcn and CD31 median fluorescence intensity (MFI) per field view. Nuclei were stained with DAPI (blue). Scale bar: 100 mum. For all charts, data represent means +- standard deviations (n = 6). Groups labeled with different lowercase letters are significantly different (p < 0.05). Fig. 7

Leptin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 2 EREG regulated glucose uptake via binding with LepR in Lep ob mice. ( A ) EREG and insulin tolerance test in Lep ob mice ( n = 5 per group) treated with a single intraperitoneal injection of insulin (0.012 IU/g BW, triangle dashed line) or EREG (80 ng/g BW, closed circles. Asterisks, significant (* p < 0.05) compared to glucose levels before EREG treatment. # Hashtag, significant difference in glucose levels 30 min after treatment with EREG or insulin. Unpaired Student's t -test. ( B ) Area under the curve (AUC) quantification of insulin (hatched bar) and EREG (black bar) tolerance tests. Unpaired Student's t -test, ns . ( C ) GTT kinetics were measured in Lep ob mice ( n = 5 per treatment) treated with a single injection of PBS (Veh, open circles) or EREG (closed circles). Student's t -test. * p < 0.05 from comparison between control and EREG treated mice at each time point. ( D ) Area under the curve (AUC) quantification of insulin (hatched bar) and EREG (black bar) tolerance tests. Unpaired Student's t -test. ( E , F ). Immunoprecipitation of LepR was performed with anti-EREG antibody using homogenates from subcutaneous fat ( C ) and visceral fat ( D ). Fat tissue was isolated from non-treated Lep ob (Veh) as well as Lep ob mice 15 min after injection of EREG (50 ng/mL).

Product Details

PA5-18522

Applications	Tested Dilution	Publications
Western Blot (WB)	1 - 2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	4 - 6 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Flow Cytometry (Flow)	10 µg/mL	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (TQDDIEKHQSDAG) corresponding to the internal amino acids of LEPR
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10977531

Product Specific Information

This antibody is predicted to react with canine based on sequence homology.

Target Information

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration.

Bioactive materials

Ma YX,Jiao K,Wan QQ,Li J,Liu MY,Zhang ZB,Qin W,Wang KY,Wang YZ,Tay FR,Niu LN

Published figure using Leptin Receptor polyclonal antibody (Product # PA5-18522) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2022

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration.

Bioactive materials

Ma YX,Jiao K,Wan QQ,Li J,Liu MY,Zhang ZB,Qin W,Wang KY,Wang YZ,Tay FR,Niu LN

Published figure using Leptin Receptor polyclonal antibody (Product # PA5-18522) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2022

Epiregulin as an Alternative Ligand for Leptin Receptor Alleviates Glucose Intolerance without Change in Obesity.

Cells

Song NJ,Lee A,Yasmeen R,Shen Q,Yang K,Kumar SB,Muhanna D,Arnipalli S,Noria SF,Needleman BJ,Hazey JW,Mikami DJ,Ortega-Anaya J,Jiménez-Flores R,Prokop J,Ziouzenkova O

Published figure using Leptin Receptor polyclonal antibody (Product # PA5-18522) in Immunoprecipitation

Wed Jan 26 00:00:00 EST 2022

Epiregulin as an Alternative Ligand for Leptin Receptor Alleviates Glucose Intolerance without Change in Obesity.

Cells

Song NJ,Lee A,Yasmeen R,Shen Q,Yang K,Kumar SB,Muhanna D,Arnipalli S,Noria SF,Needleman BJ,Hazey JW,Mikami DJ,Ortega-Anaya J,Jiménez-Flores R,Prokop J,Ziouzenkova O

Published figure using Leptin Receptor polyclonal antibody (Product # PA5-18522) in Immunoprecipitation

Wed Jan 26 00:00:00 EST 2022

Bioinformatics

Protein Aliases: B219; CD antigen CD295; CD295; CD295 antigen; HuB219; HuB219.2; LEP-R; Leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; OB receptor; OB-R

Gene Aliases: CD295; DB; diabetes; Fa; LEP-R; LEPR; Leprb; LEPRD; LEPROT; Modb1; OB-R; OB-RGRP; obese-like; obl; OBR

UniProt ID: (Human) Q13594, (Rat) Q62959, (Mouse) P48356

Entrez Gene ID: (Human) 3953, (Rat) 24536, (Mouse) 16847

Molecular Function: transmembrane signal receptor