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Lin28A/B Antibody (BS-8443R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lin28A/B Polyclonal Antibody, Unconjugated (bs-8443R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-8443R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Lin28A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein synthesis efficiency and binds to the growth and differentiation factor IGF-II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: lin-28 homolog B (C. elegans); Lin-28.2; Lin-28A; Lin-28B; Protein lin-28 homolog A; Protein lin-28 homolog B; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
Gene Aliases: 2810403D23Rik; AL024421; CSDD1; CSDD2; D030047M17Rik; ENSMUSG00000070700; Gm10299; LIN-28; Lin-28.2; lin-28A; LIN28; LIN28A; LIN28B; RGD1566408; Tex17; ZCCHC1
UniProt ID: (Human) Q9H9Z2, (Human) Q6ZN17, (Mouse) Q8K3Y3, (Mouse) Q3UZC6
Entrez Gene ID: (Human) 79727, (Human) 389421, (Rat) 500562, (Mouse) 83557, (Rat) 689054, (Mouse) 380669
regulation of transcription, DNA-templated
germ cell development
miRNA catabolic process
stem cell population maintenance
pre-miRNA processing
RNA 3'-end processing
somatic stem cell population maintenance
positive regulation of neuron differentiation
negative regulation of glial cell differentiation
positive regulation of translation
stem cell differentiation
regulation of gene silencing by miRNA
positive regulation of cell proliferation involved in kidney development
biological_process
miRNA metabolic process
gene silencing by RNA
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