Search
Agrisera
Linamarase Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
Linamarase Antibody (AS15-2862) in WB
Samples: Latex - approximately 20 µL dissolved in 200 µL of extraction buffer Leaf (cassava) - 2 leaf discs (A=78.5 mm 2), snap frozen in liquid nitrogen and homogenized with pistil in 200 µL extraction buffer 10 µL of extract, is mixed with sample buffer (Bio Rad XT), reducing agent (Bio Rad XT) and Mili Q water to a final volume of 35 µL - 30 µLl of this extract is separated on 12% Bis-Tris (Bio-Rad Criterion) gel and run at 200 V for 1 h in XT MOPS buffer. Blotting is done to PVDF membrane with Bio-Rad Criterion, 100 V/30 min. Membranes... View More
Product Details
AS15-2862
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Manihot esculenta linamarase protein sequence.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Manihot esculenta
Target Information
Linamarase is a cyanogenic beta-glucosidase encoded in cyanogenic plants (classically at the Li locus in white clover) and is localized predominantly outside the cytoplasm in the apoplast/cell wall, consistent with its role in initiating cyanogenesis only after tissue disruption brings enzyme and substrate into contact. It is synthesized as a secreted glycoprotein precursor containing an N-terminal signal peptide and multiple N-linked glycosylation sites, and the mature enzyme accumulates as an oligomeric protein composed of similar-sized subunits of roughly 60-63 kDa, with native species frequently observed as dimers/oligomers and, in cassava, multiple acidic isozymic forms. Functionally, linamarase catalyzes hydrolysis of the cyanogenic glucosides linamarin (and the related lotaustralin), releasing glucose and producing an unstable cyanohydrin that can decompose to hydrogen cyanide; this compartmentalized "enzyme in apoplast, substrate in cells" arrangement underpins a rapid, damage-activated chemical defense against herbivory and pathogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: beta-D-glucosidase; beta-glucoside glucohydrolase; cyanogenic beta-glucosidase; Li
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support