Lucifer Yellow Polyclonal Antibody, Biotin
Product Image Gallery
Figure 2 Electrophysiological profile of mature Nkx2-1 neocortical interneuron subtypes. Intrinsic electrophysiology profiles of the three observed Nkx2-1 interneuron subtypes: ( a , b ) fast spiking (FS), ( c , d ) non-fast spiking (NFS) and ( e , f ) rebound intrinsic bursting (rIB) interneuron. Scale bar (shown in a ): 200 ms, 25 mV ( a , c , e ) Threshold spike, resting membrane potential and response to hyperpolarising current injection for the various subtypes; note the burst of two or more action potentials observed in response to release of hyperpolarising current injection in the rIB interneuron ( e ). Inset: corresponding near maximal firing frequency response for the ( a ) FS, and ( c ) NFS cells; rIB interneurons showed adaptation in spike frequency similar to the ( c ) NFS subtype. ( b , d , f ) Phase plot (d V /d t versus voltage) for the cells shown in a , c and e , observed in response to suprathreshold current injection sufficient to elicit 10 spikes (20 Hz). ( g ) Silhouette plot derived from k -means (Matlab R2014a) cluster analysis with k (the number of clusters) set at 3 (left) and 4 (right plot); larger silhouette values closer to 1 are indicative of a compact cluster distinct from other clusters. ( h ) Dendrogram of hierarchical unsupervised clustering based on nine electrophysiological variables measured in 50 Nkx2-1 interneurons. The x axis represents individual cells and the y axis Euclidean distance. FS cells are shown in blue, NFS and rIB in light
Figure 3 Identification of Nkx2-1 interneuron subtypes through early postnatal development. ( a ) Percentage of Nkx2-1Cre;Z/EG EGFP+ cells expressing somatostatin (SST) across the depth of a 250-mum width column of S1BF neocortex at four time points through early development; corresponding data for ( b ) calretinin (CR) and ( c ) parvalbumin (PV; data obtained from n>=4 brains). Responses to threshold and hyperpolarizing current injection of d P5 fast spiking (FS) interneuron; ( e ) P7 non-fast spiking (NFS) interneuron; ( f ) P6 rebound intrinsic spiking interneuron (rIB), arrow, burst of action potentials observed following release from hyperpolarizing current injection; scale bar, 20 mV, 200 ms. Inset: near maximal firing frequency recordings for the corresponding cells. ( g ) Silhouette plot derived from k -means cluster analysis with k (the number of clusters) set at 2 (left), 3 (middle) and 4 (right plot). ( h - j ) Phase plot (d V /d t versus voltage) for the same cells as d - f observed in response to suprathreshold current injection sufficient to elicit 10 spikes (20 Hz). [B], biphasic rising phase; [M], monophasic rising phase. ( k ) SST expression (arrow) in the recovered Lucifer yellow (LY)-filled soma of the neuron shown in e (scale bar, 12 mum). ( l ) Relationship between phase plot profile ( x axis; M, monophasic, B, biphasic) and expression of SST in recorded immature interneurons. ( m , n ) Reconstructed morphologies of early Nkx2-1 interneurons with characte
Figure 3 Intracellular localization of mannosylated SLPs. A) Wild-type BM-DCs were incubated simultaneously with Alexa647-labeled SLPs and Alexa488-labeled OVA for 15 min. After medium chase of another 20 min, intracellular localization was determined by immunofluorescence microscopy. B) Wild-type BM-DCs were incubated with fluorochrome-labeled OVA, SLPs, Transferrin and/or Lucifer Yellow for 15 min, chased with medium for another 20 min and stained with antibodies against EEA1, LAMP1 or Lucifer Yellow. Intracellular distribution was analyzed by immunofluorescence microscopy. To analyse co-localization of OVA and SLPs with the indicated markers, the Pearson correlation coefficient (varying between -1 and +1 with -1 for perfect negative correlation, 0 for perfect absence of correlation and 1 for perfect correlation) and the Mander's overlap coefficient (varying between 0 and 1 with 0 for no overlap and +1 for perfect overlap), were calculated. Nuclei stained with DAPI are depicted in blue. PCC: Pearson Correlation Coefficient. MOC: Mander's overlap coefficient.
Figure 7 Lucifer Yellow injection to the mouse and rat claustral neurons. (A) Dark field image of the mouse slice that received Lucifer Yellow injection to the insular/claustral region. The fluorescent image of the antibody-enhanced Lucifer Yellow-filled neurons is superimposed to indicate the location of clustered injection. (B) Maximal projection stacks of confocal sections for magnified view of the insular/claustral region of (A) . Note the meshwork of dendrites that cover the insular cortex and the claustrum. (C) Another injection into the mouse slice. The pia is to the left. (D) Reconstruction of the Lucifer Yellow injected neurons shown in (C) . Different cells are shown by different colors for better representation of each cell. (E) Merged view for latexin (red) and Lucifer Yellow (green)-stained slice of the rat brain. The densely stained oval at the bottom of the insular cortex is considered as the claustrum. (F) Maximal projection stacks of confocal sections for magnified view of the insular/claustral region of (E) . Note that the insular/clautral border can be delineated by the dense fiber staining of latexin within the claustrum. Latexin + neurons were observed in a scattered manner outside the claustrum as well. (G) Magnified view of the insular/claustral border of (F) . Note the differential dendritic arborization of the claustral and insular cells. The dotted line indicates the insular/claustral border identified on the basis of latexin fiber staining. Cla, cla
PBS, pH 7.2
5mM sodium azide
Even though the cell soma of a lucifer yellow-filled neuron may be brightly stained, its finer processes can sometimes be faint and may fade rapidly or be obscured by the more intensely stained portions of the neuron. By using anti-lucifer yellow dye antibodies in conjunction with standard enzyme-mediated immunohistochemical methods one can develop a more permanent, fade-free signal for light microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lucifer Yellow Dye