Invitrogen

Lyn Monoclonal Antibody (R.677.10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (41) Lyn antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Lyn Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Tyrosine-protein kinase Lyn was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Lyn Monoclonal Antibody (R.677.10) (Product # MA5-14924) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification.

Lyn Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Lyn in paraffin-embedded Non-Hodgkin's lymphoma using a Lyn monoclonal antibody (Product # MA5-14924).

Lyn Antibody in Western Blot (WB) — Western Blot was performed using Anti-Lyn Monoclonal Antibody (R.677.10) (Product # MA5-14924) and a 58 kDa band corresponding to Tyrosine-protein kinase Lyn was observed across cell lines. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Raji (Lane 2), Jurkat (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Lyn Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with Tyrosine-protein kinase Lyn siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Lyn Monoclonal Antibody (R.677.10) (Product # MA5-14924).

Product Details

MA5-14924

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunocytochemistry (ICC/IF)

5 µg/mL

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

R.677.10

Immunogen

Synthetic peptide corresponding to the amino-terminal sequence of human Lyn

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

16.7 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10981679

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with any other Src-family members.

Target Information

Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ26625; Lck/Yes-related novel protein tyrosine kinase; lyn protein non-receptor kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

Gene Aliases: AA407514; Hck-2; JTK8; LYN; p53Lyn; p56Lyn

UniProt ID: (Human) P07948, (Mouse) P25911, (Rat) Q07014

Entrez Gene ID: (Human) 4067, (Mouse) 17096, (Rat) 81515

Function(s)

Process(es)

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding nucleotide binding protein kinase activity erythropoietin receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein complex binding