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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Lyn Antibodies

          Invitrogen

          Lyn Monoclonal Antibody (R.677.10)

          Advanced Verification
          View all (44) Lyn antibodies

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          Cite Lyn Monoclonal Antibody (R.677.10)

          • Antibody Testing Data (5)
          • Advanced Verification (1)
          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          Lyn Antibody (MA5-14924) in ICC/IF

          Immunofluorescence analysis of Tyrosine-protein kinase Lyn was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Lyn Monoclonal Antibody (R.677.10) (Product # MA5-14924) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Lyn Antibody in Immunocytochemistry (ICC/IF)
          Lyn Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody in Western Blot (WB)
          Lyn Antibody
          Lyn Monoclonal Antibody (R.677.10)

          Product Details

          MA5-14924

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Immunoprecipitation (IP)

          1:50
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          R.677.10

          Immunogen

          Synthetic peptide corresponding to the amino-terminal sequence of human Lyn
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          16.7 µg/mL

          Purification

          Affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          <0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981679

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with any other Src-family members.

          Target Information

          Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: FLJ26625; Lck/Yes-related novel protein tyrosine kinase; lyn protein non-receptor kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

          View more View less

          Gene Aliases: AA407514; Hck-2; JTK8; LYN; p53Lyn; p56Lyn

          View more View less

          UniProt ID: (Human) P07948, (Mouse) P25911, (Rat) Q07014

          View more View less

          Entrez Gene ID: (Human) 4067, (Mouse) 17096, (Rat) 81515

          View more View less

          Function(s)
          protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding nucleotide binding protein kinase activity erythropoietin receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein complex binding
          Process(es)
          B cell homeostasis regulation of cytokine production negative regulation of protein phosphorylation immune system process Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation response to hormone positive regulation of neuron projection development phosphorylation peptidyl-tyrosine phosphorylation hemopoiesis erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development lipopolysaccharide-mediated signaling pathway regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway intracellular signal transduction peptidyl-tyrosine autophosphorylation positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of STAT protein hemoglobin biosynthetic process regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of erythrocyte differentiation protein autophosphorylation cytokine secretion regulation of cytokine secretion regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid cellular response to peptide hormone stimulus regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway adaptive immune response response to sterol depletion central nervous system development blood coagulation response to toxic substance response to carbohydrate oligodendrocyte development response to organic cyclic compound viral process signal transduction by protein phosphorylation platelet activation T cell costimulation cellular response to extracellular stimulus response to insulin cellular response to heat Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to drug response to amino acid ephrin receptor signaling pathway response to axon injury negative regulation of immune response leukocyte migration JAK-STAT cascade involved in growth hormone signaling pathway chemotaxis cell migration response to peptide hormone
          It has to be done as per old AB suggested Products section.
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