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Invitrogen
MCP-1 Monoclonal Antibody (E10052)
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FIGURE: 1 / 1
MCP-1 Antibody (M2MCP1I) in WB
Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Pancreas (Lane 1), Mouse Spleen (Lane 2), Mouse Lung (Lane 3) and Mouse Cerebellum (Lane 4). The blots were probed with Anti-MCP-1 Mouse monoclonal Antibody (Product # M2MCP1I, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 25 kDa band corresponding to MCP-1 was observed across cell lines. A shift in molecular weight is due to the glyc... View More
Product Details
M2MCP1I
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
E10052
Immunogen
Recombinant human MCP-1
Conjugate
Form
Liquid
Concentration
1.00 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
M2MCP1I targets MCP-1 in ELISA applications and shows reactivity with Human samples.
The M2MCP1I immunogen is recombinant human MCP-1.
M2MCP1I detects MCP-1 which has a predicted molecular weight of approximately 9 kDa.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; monocyte chemotactic protein; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Mutations: 171:OK; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2; unnamed protein product
Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
UniProt ID: (Human) P13500
Entrez Gene ID: (Human) 6347
angiogenesis
monocyte chemotaxis
protein phosphorylation
chemotaxis
inflammatory response
immune response
humoral immune response
cytoskeleton organization
cell adhesion
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
JAK-STAT cascade
regulation of cell shape
response to bacterium
organ morphogenesis
positive regulation of gene expression
positive regulation of macrophage chemotaxis
viral genome replication
cytokine-mediated signaling pathway
sensory perception of pain
cellular homeostasis
positive regulation of cell migration
negative regulation of glial cell apoptotic process
helper T cell extravasation
chemokine (C-C motif) ligand 2 signaling pathway
negative regulation of neuron apoptotic process
astrocyte cell migration
cellular response to fibroblast growth factor stimulus
eosinophil chemotaxis
macrophage chemotaxis
positive regulation of T cell activation
positive chemotaxis
positive regulation of synaptic transmission, glutamatergic
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of calcium ion import
positive regulation of glutamate receptor signaling pathway
negative regulation of vascular endothelial cell proliferation
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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