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Invitrogen
TRA-1-81 Monoclonal Antibody (TRA-1-81), DyLight™ 550
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FIGURE: 1 / 2
TRA-1-81 Antibody (MA1-024-D550) in ICC/IF
Immunofluorescent analysis of TRA-1-81 (red) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-D550) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. Nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magni... View More
Product Details
MA1-024-D550
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TRA-1-81
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Excitation/Emission Max
556/570 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-024-D550 has been successfully used in ICC/IF applications on human samples.
Target Information
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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