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Invitrogen
Clathrin Heavy Chain Monoclonal Antibody (X22)
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FIGURE: 1 / 32
Clathrin Heavy Chain Antibody (MA1-065) in ICC/IF
Immunofluorescent analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) (Product # MA1-065) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or... View More
Product Details
MA1-065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Published species
Bovine,
Dog,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
X22
Immunogen
Purified human brain clathrin heavy chain.
Conjugate
Form
Liquid
Concentration
6 mg/mL
Amount
600 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-065 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.
MA1-065 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody recognizes a single ~180 kDa protein representing the clathrin heavy chain from bovine brain extract. Immunoprecipitation of triskelions with MA1-065 allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.
The MA1-065 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Target Information
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Clathrin heavy chain 1; Clathrin heavy chain 2; Clathrin heavy chain on chromosome 17; Clathrin heavy chain on chromosome 22; clathrin, heavy chain (Hc); clathrin, heavy polypeptide (Hc); Clathrin, heavy polypeptide D; clathrin, heavy polypeptide-like 1; clathrin, heavy polypeptide-like 2; CLH-17; CLH-22; KIAA0034; unnamed protein product
Gene Aliases: 3110065L21Rik; CHC; CHC17; CHC22; CLH-17; CLH17; CLH22; CLTC; CLTCL; CLTCL1; CLTCL2; CLTD; Hc; KIAA0034; MRD56; R74732
UniProt ID: (Human) Q00610, (Bovine) P49951, (Human) P53675, (Rat) P11442, (Mouse) Q68FD5
Entrez Gene ID: (Human) 1213, (Bovine) 281080, (Human) 8218, (Chinese hamster) 100756644, (Rat) 54241, (Mouse) 67300
RNA binding
double-stranded RNA binding
structural molecule activity
protein binding
protein kinase binding
clathrin light chain binding
low-density lipoprotein particle receptor binding
disordered domain specific binding
ubiquitin-specific protease binding
protein domain specific binding
identical protein binding
arrestin family protein binding
protein C-terminus binding
ankyrin binding
heat shock protein binding
peptide binding
poly(A) RNA binding
vesicle coat protein
mitotic cell cycle
osteoblast differentiation
intracellular protein transport
receptor-mediated endocytosis
autophagy
vesicle-mediated transport
receptor internalization
transferrin transport
retrograde transport, endosome to Golgi
clathrin coat assembly
cell division
regulation of mitotic spindle organization
clathrin coat disassembly
clathrin-mediated endocytosis
amyloid-beta clearance by transcytosis
negative regulation of hyaluronan biosynthetic process
negative regulation of protein localization to plasma membrane
anatomical structure morphogenesis
positive regulation of glucose import
Golgi organization
mitotic nuclear division
mitotic spindle assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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