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Invitrogen
NSP1 Monoclonal Antibody (32D6)
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Product Details
MA1-10029
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
32D6
Immunogen
Yeast nuclear preparation.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-10029 detects Nsp1p from S. cerevisiae samples. To confirm a high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in the RS453 strain contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for protein function, and encodes a protein that has been observed to migrate at ~85kDa on SDS-PAGE gels. Wildtype Nsp1p has a predicted MW of 86.5 kDa but has been observed to migrate on SDS gels at ~100 kDa. SDS-PAGE testing results showed wild type Nsp1p at ~108 kDa and Nsp1p RS453 at ~91 kDa, confirming specificity for the Nsp1p protein.
For western blots of yeast protein samples, use MA1-10029 diluted 1:10,000 (cell lysates) to 1:50,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try MA1-10029 diluted 1:1,000 to 1:5,000. For immunofluorescence on yeast cells, use MA1-10029 diluted 1:1,000 to 1:10,000.
Target Information
Nucleoporin NSP1 is a component of the yeast nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NSP1 plays an important role in several nuclear transport pathways including poly(A)+ RNA, tRNA, pre-ribosome, signal recognition particle (SRP), and protein transport.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nuclear pore protein NSP1; Nucleoporin NSP1; Nucleoskeletal-like protein; p110
Gene Aliases: J1207; NSP1; YJL041W
UniProt ID: (Baker's yeast) P14907
Entrez Gene ID: (Baker's yeast) 853409
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
RNA export from nucleus
tRNA export from nucleus
protein import into nucleus
NLS-bearing protein import into nucleus
cell cycle
protein transport
poly(A)+ mRNA export from nucleus
mRNA transport
ncRNA export from nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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