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Invitrogen
CD177 Monoclonal Antibody (MEM-166), PE
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FIGURE: 1 / 2
CD177 Antibody (MA1-10120) in Flow
Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD177 (MEM-166) PE Monoclonal antibody (Product # MA1-10120) using a dilution of 20 µL/100 µL of peripheral whole blood.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10120
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-166
Immunogen
Human granulocytes
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.02 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
Target Information
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell surface receptor; human neutrophil alloantigen 2a; human neutrophil antigen 2; NB1 glycoprotein; polycythemia rubra vera protein 1; unnamed protein product
Gene Aliases: HNA-2; HNA-2a; HNA2A; NB1; NB1 GP; PRV-1; PRV1
UniProt ID: (Human) Q8N6Q3
Entrez Gene ID: (Human) 57126
cell adhesion
leukocyte cell-cell adhesion
regulation of endocytosis
positive regulation of superoxide anion generation
protein localization to cell surface
positive regulation of neutrophil degranulation
cell-cell junction maintenance
neutrophil extravasation
cell-cell adhesion
neutrophil migration
regulation of integrin-mediated signaling pathway
Disclaimer
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