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Invitrogen
beta-3 Tubulin Monoclonal Antibody (2G10)
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FIGURE: 1 / 33
beta-3 Tubulin Antibody (MA1-118) in ICC/IF
Immunofluorescence analysis of beta III tubulin (green) in the ectoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09) using Gibco® KnockOut™ Serum Replacement. After four days in suspension culture, EBs were plated on Geltrex™-coated tissue culture-treated polystyrene and continuously cultured for 21 days. EB cultures... View More
Product Details
MA1-118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Guinea pig,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat
Published species
Amphibian,
Human,
Mouse,
Rabbit,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2G10
Immunogen
A synthetic peptide corresponding to amino acids 436-450 from rat neuronal specific beta-3 tubulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-118 was successfully used to detect E18 Sparague Dawley primary cortical neurons.
MA1-118 can be used for immunofluorescence analysis of beta III tubulin in the ectoderm derived from human embryonic stem cells.
Western blot analysis of MA1-118 detects an ~50 kDa protein in neuronal-type cells. In bovine, a unknown band at ~32 kDa is also detected. MA1-118 shows specificity to beta-3 Tubulin and is non-reactive to lysates from non-neuronal cell types (e.g. HeLa cell lysate).
Target Information
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta 3 tubulin; beta tubulin 3; betaIII-tubulin; class III beta-tubulin; MC1R; melanocortin receptor 1; microtubule protein; Neuron specific beta III Tubulin; Neuron-specific class III beta-tubulin; TUBB; tubulin beta; Tubulin beta-3; Tubulin beta-3 chain; Tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta, 3; tubulin, beta, 4; unnamed protein product
Gene Aliases: 3200002H15Rik; beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; I79_000194; M(beta)3; M(beta)6; mc1R; TUBB3; TUBB4
UniProt ID: (Human) Q13509, (Bovine) Q2T9S0, (Mouse) Q9ERD7, (Rat) Q4QRB4
Entrez Gene ID: (Rabbit) 100341581, (Human) 10381, (Bovine) 768070, (Mouse) 22152, (Rat) 246118, (Chinese hamster) 100760055
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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