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Invitrogen

CD8/CD28 Antibody Cocktail, FITC, PE

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Product Details

MA1-12166

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

17D8, B-23

Immunogen

human peripheral blood T lymphocytes and HPB-ALL T-cell line

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.2% carrier protein

Contains

0.08% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073423

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD8 (clone 17D8, isotype IgG1) and PE-conjugated CD28 (clone B-23, isotype IgG1).

Target Information

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells. Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD28; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8beta; cell surface glycoprotein T8 precursor; Leu-2; Leu2 T-lymphocyte antigen; Ly-3 homolog; MGC138290; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein (CD8-beta) precursor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell differentiation antigen Leu-2/T8; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell-specific homodimer surface protein precursor; T-cell-specific surface glycoprotein CD28; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen; TP44; unnamed protein product

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Gene Aliases: CD28; CD8; CD8A; CD8alpha; CD8B; CD8B1; CD8beta; IMD116; IMD123; LEU2; Ly-3; LY3; LYT3; MAL; p32; P37; Tp44

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UniProt ID: (Human) P10747, (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 940, (Human) 925, (Human) 926

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Function(s)
protein binding coreceptor activity protein kinase binding identical protein binding MHC class I protein complex binding MHC class I protein binding
Process(es)
adaptive immune response immune system process immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of immune response T cell receptor signaling pathway T cell mediated immunity cell surface receptor signaling pathway antigen processing and presentation cytotoxic T cell differentiation positive regulation of cytokine production positive regulation of inflammatory response to antigenic stimulus transcription from RNA polymerase II promoter humoral immune response positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-2 production positive regulation of interleukin-4 production CD4-positive, alpha-beta T cell proliferation T cell proliferation positive regulation of T cell proliferation regulation of T cell proliferation negative regulation of apoptotic process protein kinase B signaling negative thymic T cell selection regulatory T cell differentiation positive regulation of viral genome replication regulation of regulatory T cell differentiation positive regulation of translation positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgG isotypes positive regulation of T cell activation positive regulation of protein kinase B signaling apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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