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Invitrogen
6x-His Tag Monoclonal Antibody (4E3D10H2/E3), Alexa Fluor™ 555
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-A555-Antibody-MA1-135-A555-IF-120160323131146.jpg?time=20240507 "6x-His Tag Antibody in Immunocytochemistry (ICC/IF)")
FIGURE: 1 / 3
6x-His Tag Antibody (MA1-135-A555) in ICC/IF
Immunofluorescent analysis of HeLa cells transfected with a construct containing a HIS Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 555 conjugated HIS-tag monoclonal antibody (Product # MA1-135-A555) ... View More
-A555-Antibody-MA1-135-A555-IF-120160323131146.jpg?time=20240507)
-A555-Antibody-MA1-135-A555-IF-120160323131146.jpg?time=20240507?time=20240507 "6x-His Tag Antibody (MA1-135-A555) in ICC/IF")
Product Details
MA1-135-A555
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E3D10H2/E3
Immunogen
Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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