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Invitrogen
beta Actin Monoclonal Antibody (15G5A11/E2)
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FIGURE: 1 / 52
beta Actin Antibody (MA1-140) in ICC/IF
Immunofluorescent analysis of Beta- Actin (green) in U2OS cells untreated or treated with 1uM Actinomycin D for 20 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Beta-Actin mouse monoclonal antibody (Product # MA1-140) at a dilution of 1:2000 in blo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Published species
Dog,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
15G5A11/E2
Immunogen
Purified full-length recombinant human beta-actin expressed in E. coli
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
By Western blot, MA1-140 detects a prominent ~42kD protein in human, mouse, non-human primate, and rat whole cell lysates.
For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-X actin; A-X actin-like protein; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, beta, cytoplasmic; beta cytoskeletal actin; beta-actin; cytoplasmic beta-actin; cytoplasmic protein; I(2)-actin; melanoma X-actin; PS1TP5-binding protein 1; unnamed protein product
Gene Aliases: Actx; beta-actin; BKRNS; BNS; BRWS1; CSMH; DDS1; E430023M04Rik; PS1TP5BP1; THC8
UniProt ID: (Human) P60709, (Dog) O18840, (Mouse) P60710, (Rat) P60711
Entrez Gene ID: (Human) 60, (Dog) 403580, (Mouse) 11461, (Rat) 81822
structural constituent of cytoskeleton
protein binding
ATPase activity
kinesin binding
protein kinase binding
nitric-oxide synthase regulator activity
Tat protein binding
nucleosomal DNA binding
identical protein binding
tau protein binding
nitric-oxide synthase binding
structural constituent of postsynaptic actin cytoskeleton
transporter regulator activity
ATP binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cytoskeleton organization
microtubule nucleation
establishment or maintenance of cell polarity
axonogenesis
positive regulation of cell proliferation
substantia nigra development
regulation of mitotic metaphase/anaphase transition
adherens junction assembly
maintenance of blood-brain barrier
regulation of apoptotic process
apical protein localization
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cell motility
regulation of norepinephrine uptake
positive regulation of norepinephrine uptake
regulation of cell cycle
regulation of G0 to G1 transition
platelet aggregation
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
positive regulation of stem cell population maintenance
positive regulation of double-strand break repair via homologous recombination
regulation of G1/S transition of mitotic cell cycle
regulation of double-strand break repair
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
cellular response to electrical stimulus
regulation of protein localization to plasma membrane
circadian rhythm
response to mechanical stimulus
dynein-driven meiotic oscillatory nuclear movement
response to immobilization stress
retina development in camera-type eye
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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