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FIGURE: 1 / 10
DYKDDDDK Tag Antibody (MA1-142) in WB
Western blot was performed using Anti-DYKDDDDK Tag Monoclonal Antibody (L5) (Product # MA1-142) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3-DYKDDDDK, 40 µg (Lane 3), HA-H3-DYKDDDDK, 20 µg (Lane 4), HA-H3-DYKDDDDK, 10 µg (Lane 5), HA-H3-DYKDDDDK, 5 µg (Lane 6), DYKDDDDK-p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Produ... View More
Product Details
MA1-142
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
L5
Immunogen
Recombinant fusion proteins of mouse Langerin/CD207
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates.
Similar Epitope as the FLAG tag from Sigma.
The MA1-142 immunogen is recombinant fusion proteins of mouse Langerin/CD207, which contained a flexible linker sequence from E. coli OmpF and a FLAG epitope. (J Immunol Methods. 2008 Feb 29;331(1-2):27-38. Epub 2007 Nov 26.)
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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