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Invitrogen

BCR-ABL Monoclonal Antibody (7C6)

Advanced Verification
1 Published Figure
2 References

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BCR-ABL Monoclonal Antibody (7C6)

Product Details

MA1-153

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-

Immunoprecipitation (IP)

5 µg
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7C6

Immunogen

Bcr686 thyroglobulin conjugate corresponding to human BCR sequence 686-696 (SSINEEITPRRQS)
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536861

Product Specific Information

Western blot analysis of MA1-153 detects ~130-160 kDa and a 210-250 kDa protein in K562 cells. In addition to BCR-ABL, BCR and ABL are also expressed in these cells and full length BCR is also detected by the antibody. For detection purposes, non-reducing conditions resulted in enhanced detection of BCR-ABL.

Target Information

A reciprocal translocation between chromosomes 22 and 9 produces the BCR-ABL fusion oncoprotein, which is found in patients with chronic myelogenous leukemia (CML). Although there are various versions, p210 is the most commonly found BCR-ABL form. The fusion to BCR, results in deregulated/constitutively active tyrosine kinase activity of BCR-ABL which is sufficient of oncogenic transformation of myeloid cells. Inhibition of BCR-ABL oncogenic activity by the ABL-kinase inhibitor STI-571 (also known as gleevec, imatinib) became the first and best example for successful targeted therapy of cancer, and specifically CML. In recent years, several mutations (such as the T351I mutation) were found in CML patients that resulted in STI-571 resistance and the need for additional drug development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bcr; BCR, RhoGEF and GTPase activating protein; bcr/able fusion protein; BCR/FGFR1 chimera protein; breakpoint cluster region; breakpoint cluster region homolog; Breakpoint cluster region protein; FGFR1/BCR chimera protein; protein kinase; Renal carcinoma antigen NY-REN-26; unnamed protein product

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Gene Aliases: 5133400C09Rik; AI561783; AI853148; ALL; BCR; BCR1; CML; D22S11; D22S662; Kiaa3017; mKIAA3017; PHL

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UniProt ID: (Human) P11274, (Mouse) Q6PAJ1

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Entrez Gene ID: (Human) 613, (Mouse) 110279

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Function(s)
nucleotide binding protein serine/threonine kinase activity protein tyrosine kinase activity guanyl-nucleotide exchange factor activity GTPase activator activity protein binding ATP binding kinase activity transferase activity protein serine kinase activity Rho guanyl-nucleotide exchange factor activity enzyme binding G-protein modulator
Process(es)
negative regulation of cellular extravasation renal system process protein phosphorylation phagocytosis signal transduction small GTPase mediated signal transduction brain development cell migration actin cytoskeleton organization keratinocyte differentiation response to lipopolysaccharide regulation of Rho protein signal transduction intracellular signal transduction inner ear morphogenesis regulation of vascular permeability neutrophil degranulation negative regulation of neutrophil degranulation focal adhesion assembly homeostasis of number of cells negative regulation of inflammatory response positive regulation of phagocytosis modulation of synaptic transmission neuromuscular process controlling balance regulation of small GTPase mediated signal transduction establishment of localization in cell regulation of cell cycle definitive hemopoiesis negative regulation of respiratory burst negative regulation of blood vessel remodeling intracellular protein transmembrane transport cellular response to lipopolysaccharide activation of GTPase activity macrophage migration negative regulation of macrophage migration negative regulation of reactive oxygen species metabolic process phosphorylation negative regulation of cell migration positive regulation of GTPase activity protein autophosphorylation platelet-derived growth factor receptor signaling pathway
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