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Invitrogen
Cyclin A2 Monoclonal Antibody (E23.1)
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FIGURE: 1 / 14
Cyclin A2 Antibody (MA1-154) in ICC/IF
Immunofluorescence analysis of Cyclin A2 was performed using 70% confluent log phase HeLa cells treated with Camptothecin (100nM, 16 hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin A2 Monoclonal Antibody (Product # MA1-154) at 1:50 dilution in 0... View More
Product Details
MA1-154
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
E23.1
Immunogen
Bovine Cyclin A fusion protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-154 detects Cyclin A in mammalian samples and has been successfully used in Immunohistochemistry (Frozen and Paraffin), Immunofluorescence, Immunoprecipitation and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-154 show the accumulation of a prominent band at ~50 kDa in camptothecin and hydroxyurea treated cells. In Immunofluorescence applications, MA1-154 shows nuclear translocation of Cyclin A in G2/M arrested cells as a result of camptothecin treatment.
Target Information
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCNA2; Cyclin A; Cyclin A2 (Cyclin A); Cyclin-A; Cyclin-A2; unnamed protein product
Gene Aliases: AA408589; Ccn-1; CCN1; CCNA; CCNA2; Cyca; CycA2
UniProt ID: (Human) P20248, (Bovine) P30274, (Mouse) P51943
Entrez Gene ID: (Human) 890, (Bovine) 281667, (Rat) 114494, (Mouse) 12428
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
regulation of DNA replication
transcription, DNA-templated
Ras protein signal transduction
organ regeneration
response to estradiol
response to glucagon
cellular response to platelet-derived growth factor stimulus
post-translational protein modification
cellular response to leptin stimulus
mitotic cell cycle phase transition
cell cycle G1/S phase transition
positive regulation of transcription, DNA-templated
positive regulation of fibroblast proliferation
cell division
cellular response to cocaine
cellular response to luteinizing hormone stimulus
cellular response to estradiol stimulus
cellular response to hypoxia
cellular response to nitric oxide
cochlea development
cellular response to insulin-like growth factor stimulus
positive regulation of DNA biosynthetic process
regulation of cyclin-dependent protein serine/threonine kinase activity
regulation of G2/M transition of mitotic cell cycle
cell cycle
mitotic nuclear division
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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