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Invitrogen

HIF-1 beta Monoclonal Antibody (H1beta234)

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HIF-1 beta Monoclonal Antibody (H1beta234)

Product Details

MA1-16512

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Immunoprecipitation (IP)

Assay-Dependent
-

ChIP assay (ChIP)

1:10-1:500
-

ChIP-sequencing (ChIP-Seq)

Assay-Dependent
-

Gel Shift (EMSA)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Bovine, Ferret, Human, Mouse, Non-human primate, Sheep, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

H1beta234

Immunogen

Fusion protein containing amino acids 496-789 of human HIF-1 beta.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2059442

Product Specific Information

Suggested positive control: antigen standard for ARNT (transient overexpression lysate).

Target Information

HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ARNT; ARNT protein; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; basic helix-loop-helix transcription factor; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; hif 1; HIF-1 beta; HIF-1-beta; HIF1 beta; HIF1-beta; hypoxia inducible factor 1 subunit beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; unnamed protein product

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Gene Aliases: ARNT; ARNT1; BHLHE2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714

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UniProt ID: (Bovine) Q9BE97, (Human) P27540, (Rat) P41739, (Mouse) P53762

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Entrez Gene ID: (Bovine) 281010, (Sheep) 443285, (Human) 405, (Rat) 25242, (Mouse) 11863

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Function(s)
transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity sequence-specific double-stranded DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding aryl hydrocarbon receptor binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity signal transducer activity aryl hydrocarbon receptor activity transcription factor binding enhancer binding basic helix-loop-helix transcription factor
Process(es)
regulation of transcription, DNA-templated response to hypoxia embryonic placenta development positive regulation of endothelial cell proliferation regulation of transcription from RNA polymerase II promoter positive regulation of vascular endothelial growth factor production cell differentiation intracellular receptor signaling pathway positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of protein sumoylation cellular response to oxidative stress positive regulation of erythrocyte differentiation positive regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone biosynthetic process negative regulation of inflammatory response intestinal epithelial structure maintenance transcription, DNA-templated transcription from RNA polymerase II promoter signal transduction response to toxic substance regulation of transcription from RNA polymerase II promoter in response to oxidative stress regulation of nucleic acid-templated transcription
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