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Invitrogen
Blimp-1 Monoclonal Antibody (3H2-E8)
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FIGURE: 1 / 4
Blimp-1 Antibody (MA1-16874) in ICC/IF
Immunocytochemistry analysis of Blimp-1 in A431 cells. Samples were incubated with Blimp-1 monoclonal antibody (Product # MA1-16874) using a dilution of 10 µg/mL overnight at 4°C followed by anti-mouse IgG Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
MA1-16874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Published species
Mouse,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3H2-E8
Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).
Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.
This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.
Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; BLIMP-1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; Positive regulatory domain I-binding factor 1; PR domain 1; PR domain containing 1 with ZNF domain isoform 2; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-BF1 alpha; PRDI-BF1 beta; PRDI-binding factor-1; RP1-134E15.1; unnamed protein product
Gene Aliases: b2b1765Clo; BLIMP-1; BLIMP1; PRDI-BF1; PRDM1; ZNFPR1A1
UniProt ID: (Human) O75626, (Mouse) Q60636
Entrez Gene ID: (Pig) 100154284, (Human) 639, (Mouse) 12142
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
methyltransferase activity
zinc ion binding
transferase activity
metal ion binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
morphogenesis of a branching structure
kidney development
embryonic placenta development
maternal placenta development
adaptive immune response
immune system process
heart valve development
cardiac septum development
ventricular septum development
regulation of transcription from RNA polymerase II promoter
germ cell development
post-embryonic development
gene expression
positive regulation of gene expression
negative regulation of gene expression
methylation
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
aorta development
regulation of cell proliferation
eye photoreceptor cell development
innate immune response
cell fate commitment
artery morphogenesis
cellular developmental process
regulation of NK T cell differentiation
retinal bipolar neuron differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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