Search
Invitrogen
Blimp-1 Monoclonal Antibody (3H2-E8)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 4
Blimp-1 Antibody (MA1-16874) in ICC/IF
Immunocytochemistry analysis of Blimp-1 in A431 cells. Samples were incubated with Blimp-1 monoclonal antibody (Product # MA1-16874) using a dilution of 10 µg/mL overnight at 4°C followed by anti-mouse IgG Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-16874
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig
Published species
Mouse,
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3H2-E8
Immunogen
A fragment of mouse Blimp-1 corresponding to residues 199-409.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western Blot, this antibody recognizes a band at ~98 kDa and may recognize one at ~80 kDa (the beta form).
Antigen retrieval is recommended (EDTA buffer, microwave) prior to IHC on paraffin tissues.
This antibody does not immunoprecipitate.For IHC and ICC/IF a dilution of 1:200 is recommended with tyramide amplification; this antibody demonstrates nuclear staining.
Suggested positive control: antigen standard for PRDM1 (transient overexpression lysate), P3X (murine plasmocytoma) cell lysates.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B lymphocyte induced maturation protein; B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain 1; PR domain containing 1 with ZNF domain isoform 2; PR domain containing 1, with ZNF domain; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-BF1 alpha; PRDI-BF1 beta; PRDI-binding factor-1; RP1-134E15.1; transcriptional repressor; binds specifically to the PRDI element in the promoter of the beta-interferon gene; drives the maturation of B-lymphocytes into Ig secreting cells; unnamed protein product
Gene Aliases: b2b1765Clo; BLIMP-1; BLIMP1; PRDI-BF1; ZNFPR1A1
UniProt ID: (Human) O75626, (Mouse) Q60636
Entrez Gene ID: (Pig) 100154284, (Human) 639, (Mouse) 12142
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
histone deacetylase binding
sequence-specific DNA binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
gene expression
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
cell fate commitment
cellular developmental process
regulation of NK T cell differentiation
in utero embryonic development
morphogenesis of a branching structure
kidney development
embryonic placenta development
maternal placenta development
heart valve development
cardiac septum development
ventricular septum development
germ cell development
post-embryonic development
positive regulation of gene expression
negative regulation of gene expression
peptidyl-arginine methylation
negative regulation of B cell proliferation
negative regulation of lipopolysaccharide-mediated signaling pathway
aorta development
regulation of cell proliferation
eye photoreceptor cell development
positive regulation of B cell differentiation
artery morphogenesis
retinal bipolar neuron differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support