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Invitrogen
CD18 Monoclonal Antibody (MEM-48)
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Product Details
MA1-19007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-48
Immunogen
Leucocytes from a patient suffering from a LGL type leukemia.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
It will not cross-react with canine.
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Western Blot: non-reducing conditions; Functional Application: Induction.
Target Information
CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); leukocyte adhesion protein beta-subunit precursor; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; putative; unnamed protein product
Gene Aliases: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
UniProt ID: (Human) P05107
Entrez Gene ID: (Human) 3689
microglial cell activation
leukocyte migration involved in inflammatory response
receptor-mediated endocytosis
phagocytosis
phagocytosis, engulfment
apoptotic process
inflammatory response
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell-cell signaling
regulation of cell shape
neutrophil chemotaxis
receptor internalization
positive regulation of superoxide anion generation
cell adhesion mediated by integrin
heterotypic cell-cell adhesion
endodermal cell differentiation
receptor clustering
positive regulation of neutrophil degranulation
endothelial cell migration
cellular extravasation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
negative regulation of dopamine metabolic process
regulation of peptidyl-tyrosine phosphorylation
cellular response to low-density lipoprotein particle stimulus
positive regulation of protein targeting to membrane
beta-amyloid clearance
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of leukocyte adhesion to vascular endothelial cell
neutrophil migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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