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Cytokeratin 18 Monoclonal Antibody (C-04)
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FIGURE: 1 / 10
Cytokeratin 18 Antibody (MA1-19039) in ICC/IF
Immunofluorescence analysis of Cytokeratin 18 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes, blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 18 Monoclonal Antibody (C-04) (Product # MA1-19039) at a concentration of 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then l... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-19039
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mammal,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C-04
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
Perform Western blot under non-reducing conditions.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; cytokeratin endo B; cytokeratin-18; Cytokeratin18; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; keratin D; Keratin18; KRT 18; ORF; PIG46; type I cytoskeletal 18; unnamed protein product
Gene Aliases: CK-18; CK18; CYK18; K18; Krt1-18
UniProt ID: (Human) P05783, (Rat) Q5BJY9, (Mouse) P05784
Entrez Gene ID: (Human) 3875, (Rat) 294853, (Mouse) 16668
response to fructose
response to nutrient levels
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
intermediate filament organization
hepatocyte differentiation
mesenchymal stem cell differentiation
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cholangiocyte apoptotic process
anatomical structure morphogenesis
cell-cell adhesion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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