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Invitrogen
LCK Monoclonal Antibody (LCK-01)
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FIGURE: 1 / 7
LCK Antibody (MA1-19197) in WB
Western blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01) Monoclonal antibody (Product # MA1-19197). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck; Lane 2: HEK-293T cell line transfected with Lck; Lane 3: HEK-293T cell line (non-transfected).
Product Details
MA1-19197
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LCK-01
Immunogen
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).
This antibody will not cross-react with mouse.
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck; unnamed protein product
Gene Aliases: IMD22; LCK; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
nucleotide binding
phosphotyrosine binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
receptor binding
protein binding
ATP binding
phospholipase activator activity
kinase activity
transferase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
T cell receptor binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phospholipase binding
phosphatidylinositol 3-kinase binding
ATPase binding
protein antigen binding
immune response-activating cell surface receptor signaling pathway
protein phosphorylation
cellular zinc ion homeostasis
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
response to xenobiotic stimulus
positive regulation of gene expression
peptidyl-tyrosine phosphorylation
hemopoiesis
platelet activation
T cell differentiation
T cell costimulation
positive regulation of heterotypic cell-cell adhesion
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
Fc-gamma receptor signaling pathway
T cell activation
gamma-delta T cell differentiation
positive regulation of MAPK cascade
positive regulation of gamma-delta T cell differentiation
regulation of regulatory T cell differentiation
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of T cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
leukocyte migration
release of sequestered calcium ion into cytosol
positive regulation of multicellular organismal process
regulation of lymphocyte activation
CD27 signaling pathway
positive regulation of leukocyte cell-cell adhesion
positive regulation of intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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