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Invitrogen

Estrogen Receptor beta Monoclonal Antibody (14C8)

1 Published Figure
3 References
View all (45) Estrogen Receptor beta antibodies

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Estrogen Receptor beta Monoclonal Antibody (14C8)

Product Details

MA1-23217

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ChIP assay (ChIP)

Assay-dependent
-

Dot blot (DB)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

14C8

Immunogen

Amino acids 1-153 of human ER-beta expressed in E. coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.23 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_558839

Product Specific Information

For immunostaining, strong ER beta staining in epithelial cell nuclei is observed, with weak to moderate staining seen in surrounding stromal and endothelial cell nuclei, and sporadic light cytoplasmic staining. It has also been reported that this antibody has been used to detect ERb in colon and ovarian tumors. Positive control of recombinant fusion protein.

Target Information

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ER; ER beta; ER-beta; ERbeta 4; Estrogen receptor beta; estrogen receptor beta 2; estrogen receptor beta 4; nuclear receptor; Nuclear receptor subfamily 3 group A member 2; oestrogen receptor beta; splice variant; unnamed protein product

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Gene Aliases: ER-BETA; ER[b]; Erb; ERbeta; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2; ODG8

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UniProt ID: (Human) Q92731, (Mouse) O08537

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Entrez Gene ID: (Human) 2100, (Mouse) 13983

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding protein binding zinc ion binding lipid binding enzyme binding estrogen receptor activity estrogen response element binding sequence-specific DNA binding metal ion binding receptor antagonist activity core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding drug binding hormone binding peroxisome proliferator activated receptor binding estradiol binding steroid hormone binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ovarian follicle development behavioral fear response neuron migration transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter brain development learning or memory negative regulation of cell proliferation hormone-mediated apoptotic signaling pathway intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to testosterone regulation of cell proliferation positive regulation of apoptotic process steroid hormone mediated signaling pathway regulation of neuron apoptotic process estrous cycle positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of behavior negative regulation of smooth muscle cell proliferation negative regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell proliferation uterus development vagina development negative regulation of cell death prostate gland epithelium morphogenesis epithelial cell maturation involved in prostate gland development negative regulation of androgen receptor signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of neuron death negative regulation of feeding behavior negative regulation of reactive oxygen species metabolic process regulation of transcription from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of cell growth cellular response to estradiol stimulus cellular response to oxygen-containing compound
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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