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Invitrogen
MDM2 Monoclonal Antibody (MD-219)
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FIGURE: 1 / 2
MDM2 Antibody (MA1-24643) in WB
Western blot was performed using Anti-MDM2 Monoclonal Antibody (MD-219) (Product # MA1-24643) and a 55 kDa band corresponding to MDM2 was observed across cell lines tested and increased up on LPS treatment in RAW 264.7. Whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), C2C12 (Lane 2), NMuMG (Lane 3), RAW 264.7 (Lane 4) and RAW 264.7 treated with LPS (0.1 µg/mL for 10 hr) (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane ... View More
Product Details
MA1-24643
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MD-219
Immunogen
Recombinant mouse MDM2.
Conjugate
Form
Liquid
Concentration
2.2 mg/mL
Amount
110 µg
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Double minute 2 protein; E3 ubiquitin-protein ligase Mdm2; HGNC:6973; human homolog of; MDM2 variant FB28; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2
Gene Aliases: 1700007J15Rik; AA415488; Mdm-2; Mdm2
UniProt ID: (Mouse) P23804
Entrez Gene ID: (Mouse) 17246
p53 binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
enzyme binding
ubiquitin protein ligase binding
identical protein binding
peroxisome proliferator activated receptor binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
scaffold protein binding
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
traversing start control point of mitotic cell cycle
positive regulation of gene expression
negative regulation of gene expression
negative regulation of protein processing
protein ubiquitination
peptidyl-lysine modification
protein catabolic process
protein destabilization
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein localization to nucleus
regulation of protein catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
establishment of protein localization
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
positive regulation of protein export from nucleus
negative regulation of cell cycle arrest
negative regulation of signal transduction by p53 class mediator
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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