Search
Invitrogen
Blood Group Lewis A Monoclonal Antibody (SPM 522)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
MA1-39426
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SPM 522
Immunogen
Mucin isolated from an ovarian cyst
Conjugate
Form
Liquid
Purification
Protein A/G
Storage buffer
PBS, pH 7.6, with 1% BSA
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.
Target Information
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase 3; 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3; 4-fucosyltransferase; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha-(1,3/1,4)-fucosyltransferase; Alpha-3-fucosyltransferase FUT3; Blood group Lewis alpha-4-fucosyltransferase; Fucosyltransferase 3; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group); Fucosyltransferase III; FucT-III; galactoside 3(4)-L-fucosyltransferase; Lewis FT; Lewis Y; unnamed protein product
Gene Aliases: CD174; FT3B; FucT-III; FUT3; LE; Les
UniProt ID: (Human) P21217
Entrez Gene ID: (Human) 2525
protein binding
fucosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity
alpha-(1->3)-fucosyltransferase activity
protein glycosylation
protein N-linked glycosylation
protein O-linked glycosylation
lipid metabolic process
sphingolipid metabolic process
ceramide metabolic process
oligosaccharide metabolic process
oligosaccharide biosynthetic process
cell-cell recognition
positive regulation of cell-cell adhesion
regulation of cell migration
fucosylation
regulation of cell proliferation
macromolecule glycosylation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support