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Invitrogen
APE1 Monoclonal Antibody (13B 8E5C2)
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FIGURE: 1 / 21
APE1 Antibody (MA1-440) in ICC/IF
Immunofluorescent analysis of APE1 (green) showing staining in the in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an APE1 monoclonal antibody (Product # MA1-440) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conju... View More
Product Details
MA1-440
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
13B 8E5C2
Immunogen
Full-length recombinant human APE protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non human primate, canine, mouse and rat samples.
MA1-440 has been successfully used in Western blot, immunohistochemical, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate. Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining.
The MA1-440 immunogen is full-length, recombinant human APE protein.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1
Gene Aliases: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1
UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138
Entrez Gene ID: (Human) 328, (Dog) 482558, (Mouse) 11792, (Rat) 79116
DNA binding
damaged DNA binding
double-stranded telomeric DNA binding
transcription coactivator activity
transcription corepressor activity
DNA-(apurinic or apyrimidinic site) lyase activity
endonuclease activity
endodeoxyribonuclease activity
RNA-DNA hybrid ribonuclease activity
phosphodiesterase I activity
uracil DNA N-glycosylase activity
protein binding
phosphoric diester hydrolase activity
double-stranded DNA exodeoxyribonuclease activity
double-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
oxidoreductase activity
site-specific endodeoxyribonuclease activity, specific for altered base
chromatin DNA binding
protein complex binding
poly(A) RNA binding
metal ion binding
NF-kappaB binding
RNA binding
nuclease activity
endoribonuclease activity
exonuclease activity
hydrolase activity
lyase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
DNA repair
base-excision repair
DNA recombination
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
aging
negative regulation of smooth muscle cell migration
regulation of mRNA stability
cell redox homeostasis
positive regulation of DNA repair
oxidation-reduction process
cellular response to hydrogen peroxide
DNA demethylation
nucleic acid phosphodiester bond hydrolysis
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of G1/S transition of mitotic cell cycle
regulation of nucleic acid-templated transcription
telomere maintenance
base-excision repair, base-free sugar-phosphate removal
response to drug
cellular response to cAMP
cellular response to peptide hormone stimulus
negative regulation of nucleic acid-templated transcription
DNA catabolic process, endonucleolytic
DNA catabolic process, exonucleolytic
response to organonitrogen compound
cellular response to organonitrogen compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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