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Invitrogen
c-Kit Monoclonal Antibody (ACK4)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-70079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
ACK4
Immunogen
IL-3 dependent mast cells derived from WB- +/+ mice
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: belly-spot; c-kit proto-oncogene protein; ckit; dominant spotting; Dominant white spotting; kit oncogene; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; tyrosine kinase; tyrosine-protein kinase Kit; unnamed protein product
Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; SCO1; SCO5; SOW3; Ssm; Tr-kit; W
UniProt ID: (Mouse) P05532
Entrez Gene ID: (Mouse) 16590
ovarian follicle development
hematopoietic progenitor cell differentiation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
immature B cell differentiation
mast cell chemotaxis
myeloid leukocyte differentiation
positive regulation of cytokine production involved in immune response
positive regulation of dendritic cell cytokine production
protein phosphorylation
glycosphingolipid metabolic process
chemotaxis
response to stress
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
germ cell development
spermatogenesis
spermatid development
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
gonad development
visual learning
male gonad development
response to radiation
positive regulation of gene expression
cell migration
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
lamellipodium assembly
actin cytoskeleton organization
hemopoiesis
cell differentiation
B cell differentiation
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
positive regulation of mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
ectopic germ cell programmed cell death
intracellular signal transduction
hematopoietic stem cell migration
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of STAT protein
negative regulation of programmed cell death
mast cell degranulation
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
pigmentation
tongue development
leukocyte activation
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
response to cadmium ion
protein autophosphorylation
developmental pigmentation
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
regulation of developmental process
detection of mechanical stimulus involved in sensory perception of sound
cell chemotaxis
mast cell differentiation
melanocyte migration
melanocyte adhesion
positive regulation of pyloric antrum smooth muscle contraction
positive regulation of colon smooth muscle contraction
positive regulation of small intestine smooth muscle contraction
positive regulation of vascular smooth muscle cell differentiation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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