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Invitrogen
CD3e Monoclonal Antibody (CA17.2A12)
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Product Details
MA1-74203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CA17.2A12
Immunogen
Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes.
Target Information
The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD3 epsilon; CD3 TCR complex; CD3d molecule, delta (CD3-TCR complex); CD3E antigen, epsilon polypeptide (TiT3 complex); CD3g molecule, gamma (CD3-TCR complex); FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex
UniProt ID: (Dog) P27597
Entrez Gene ID: (Dog) 611571, (Dog) 479419, (Dog) 442981, (Dog) 489383
positive regulation of cell-matrix adhesion
adaptive immune response
positive regulation of T cell anergy
negative regulation of gene expression
dendrite development
cerebellum development
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
positive regulation of cell-cell adhesion mediated by integrin
negative thymic T cell selection
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
apoptotic signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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