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Invitrogen
CD16 Monoclonal Antibody (G7), PE
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Product Details
MA1-80267
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Published species
Pig
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
G7
Immunogen
Porcine peripheral blood leucocytes.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Clone G7 has been reported to block PBL-mediated antibody-dependent cell cytotoxicity (ADCC) and partially inhibit PMN-mediated ADCC.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III.
Target Information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD32; CD32 receptor 2; CDw32; cytolytic trigger molecule G7; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FcgammaRIII a.1; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-B; RP11-5K23.1
Gene Aliases: FCGR3; FCGR3B
Entrez Gene ID: (Pig) 397684
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