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Invitrogen
EIF2S1 Monoclonal Antibody (5A5)
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FIGURE: 1 / 10
EIF2S1 Antibody (MA1-079) in ICC/IF
Immunofluorescent analysis of eIF2S1 (green) showing positive staining in the cytoplasm of COS7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an eIF2S1 monoclonal antibody (Product # MA1-079) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyL... View More
Product Details
MA1-079
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5A5
Immunogen
Full length human EIF2S1 produced in HEK293T cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-079 has been successfully used in Western blot, immunohistochemistry, immunocytochemisty and immunofluorescent applications and reacts with human, monkey, and rat samples.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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