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Invitrogen
STEP Monoclonal Antibody (23E5)
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FIGURE: 1 / 2
STEP Antibody (MA1-158) in WB
Western blot analysis of STEP was performed by loading 25 µg of Wild Type (WT, right lane) and STEP Knockout (KO, left lane) rat striatum lysates, and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. STEP was detected at ~61 kD and ~46 kD in WT sample using a STEP monoclonal antibody (Product # MA1-158) at a concentr... View More
Product Details
MA1-158
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
23E5
Immunogen
A synthetic peptide of STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
Product Specific Information
By Western blot, MA1-158 detects both STEP 46 and STEP 61. By IP, STEP61 was preferentially pulled down, along with a non-specific protein at ~50kDa.
Rat brain lysate can be used as positive control.
Target Information
Reversible phosphorylation of tyrosine residues is a key regulatory mechanism for numerous cellular events such as proliferation, differentiation, gene expression and migration. Abnormalities in tyrosine phosphorylation play a role in the pathogenesis of numerous inherited or acquired human diseases from cancer to immune deficiencies. There are at least 107 genes coding for PTPs (protein tyrosine phosphatases) in the human genome. All known PTPs contain a highly conserved 12 amino acid catalytic domain and are further distinguished on the basis of additional structural motifs. The receptor-like PTPs exhibit an extracellular domain, a single transmembrane and one or two intracellular phosphatase domains. The nonreceptor cytoplasmic PTPs contain a single phosphatase domain and additional amino acid sequences that are homologous to motifs found in other classes of proteins. Protein tyrosine phosphatases PTPN5 (STEP), PTPRR and PTPN7 comprise a family of phosphatases that specifically inactivate MAPKs (mitogen-activated protein kinases). There are multiple forms of STEP in the adult rat brain which show differential enrichment in brain regions implicated in aspects of cognitive, affective, and motor behaviors. Some of the STEP isoforms are generated through alternative splicing of a single STEP gene and each has unique intracellular targets and functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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