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Invitrogen
HSP60 Monoclonal Antibody (4B9/89)
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FIGURE: 1 / 23
HSP60 Antibody (MA3-012) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 60 (Hsp60, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp60 monoclonal antibody (Product # MA3-012), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 3... View More
Product Details
MA3-012
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Bacteria,
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
4B9/89
Immunogen
Human placental HSP60.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-012 detects heat shock protein 60 kDa (HSP60) from human, mouse, rat, and primate cells, tissues and recombinant protein preparations.
MA3-012 has been successfully used in Western blot, flow cytometry, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a single 58 kDa protein representing HSP60 from human blood samples. Immunocytochemical staining of HSP60 in B-SC-1 cells with MA3-012 yields a pattern consistent with the mitochondrial localization of HSP60.
The MA3-012 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; chaperonin; chaperonin (HSP60); Chaperonin 60; CPN60; epididymis secretory sperm binding protein; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_1; heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_2; heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_4; Heat shock protein; Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; unnamed protein product
Gene Aliases: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; SPG13
UniProt ID: (Human) P10809, (Mouse) P63038, (Rat) P63039
Entrez Gene ID: (Human) 3329, (Mouse) 15510, (Rat) 63868
nucleotide binding
lipopolysaccharide binding
p53 binding
DNA replication origin binding
single-stranded DNA binding
RNA binding
double-stranded RNA binding
protein binding
ATP binding
high-density lipoprotein particle binding
isomerase activity
ATPase activity
enzyme binding
ubiquitin protein ligase binding
apolipoprotein binding
apolipoprotein A-I binding
unfolded protein binding
chaperone binding
cysteine-type endopeptidase activator activity
ATP-dependent protein folding chaperone
protease binding
protein complex binding
insulin binding
poly(A) RNA binding
protein heterodimerization activity
misfolded protein binding
positive regulation of cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
'de novo' protein folding
response to unfolded protein
apoptotic mitochondrial changes
response to cold
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
mitochondrial unfolded protein response
protein refolding
B cell proliferation
T cell activation
B cell activation
positive regulation of macrophage activation
negative regulation of apoptotic process
adhesion of symbiont to host
protein import into mitochondrial intermembrane space
isotype switching to IgG isotypes
protein stabilization
positive regulation of T cell activation
chaperone-mediated protein complex assembly
protein maturation
interaction with symbiont
cellular response to interleukin-7
negative regulation of execution phase of apoptosis
positive regulation of execution phase of apoptosis
detection of misfolded protein
B cell cytokine production
activation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of inflammatory response
chaperone mediated protein folding requiring cofactor
response to hypoxia
response to heat
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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