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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GSK3 alpha/beta Antibodies

          Invitrogen

          GSK3 alpha/beta Monoclonal Antibody (4G-1E)

          Advanced Verification
          1 Published Figure
          3 References
          View all (19) GSK3 alpha/beta antibodies

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          Cite GSK3 alpha/beta Monoclonal Antibody (4G-1E)

          • Antibody Testing Data (7)
          • Published Figures (1)
          • Advanced Verification (1)
          GSK3 alpha/beta Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GSK3 alpha/beta Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          GSK3 alpha/beta Antibody (MA3-038) in ICC/IF

          Immunofluorescent analysis of GSK-3 (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a GSK-3 monoclonal antibody (Product # MA3-038) at a dilution of 1:1000 in staining buffer for 1 hour at room temperature... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          GSK3 alpha/beta Antibody in Immunocytochemistry (ICC/IF)
          GSK3 alpha/beta Antibody in Western Blot (WB)
          GSK3 alpha/beta Antibody in Western Blot (WB)
          GSK3 alpha/beta Antibody in Western Blot (WB)
          GSK3 alpha/beta Antibody in Flow Cytometry (Flow)
          GSK3 alpha/beta Antibody in Immunoprecipitation (IP)
          GSK3 alpha/beta Antibody in RNA Immunoprecipitation (RIP)
          GSK3 alpha/beta Antibody in Western Blot (WB)
          GSK3 alpha/beta Antibody
          GSK3 alpha/beta Monoclonal Antibody (4G-1E)

          Product Details

          MA3-038

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:1,000
          -

          Flow Cytometry (Flow)

          1:100
          -

          Immunoprecipitation (IP)

          Assay-Dependent
          -

          RNA Immunoprecipitation (RIP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Dog, Fruit fly, Human, Mouse, Non-human primate, Rat

          Published species

          Fruit fly, Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4G-1E

          Immunogen

          Synthetic peptide corresponding to residues 203-219 of drosophila GSK-3 coupled to ovalbumin: KQLLHGEPNVSYICSRY
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2608651

          Product Specific Information

          MA3-038 detects GSK3 alpha/beta in Human, Mouse, Rat Canine, and Non-human primate samples.

          Immunogen is a synthetic peptide corresponding to residues 203-219 of drosophila GSK-3, which is highly conserved across multiple species.

          Target Information

          Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AT21229p; CG31003-PA; CG5182-PA; FA; Factor A; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3beta isoform; gskt-PA; mojoless; Pk34A-PA; Protein gasket; Putative glycogen synthase kinase-3 homolog; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

          View more View less

          Gene Aliases: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; BcDNA:AT21229; BEST:GH16447; C86142; CG11338; CG31003; CG5182; CT4237; Dm Gskt; Dmel\CG31003; Dmel\CG5182; Dmel_CG31003; Dmel_CG5182; GSK-3; GSK-3beta; GSK3; GSK3A; GSK3B; Gskt; mjl; NEST:bs22e06; NGSK

          View more View less

          UniProt ID: (Human) P49840, (Human) P49841, (Rat) P18265, (Mouse) Q2NL51, (Mouse) Q9WV60, (Rat) P18266, (Fruit fly) P83101

          View more View less

          Entrez Gene ID: (Human) 2931, (Dog) 484476, (Human) 2932, (Dog) 478575, (Rat) 50686, (Mouse) 606496, (Mouse) 56637, (Rat) 84027, (Fruit fly) 34705, (Fruit fly) 318552

          View more View less

          Function(s)
          protein serine/threonine kinase activity protein binding ATP binding protein kinase A catalytic subunit binding tau-protein kinase activity RNA polymerase II transcription factor binding p53 binding protein kinase activity integrin binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups transcription factor binding cyclin-dependent protein serine/threonine kinase activity RNA polymerase II carboxy-terminal domain kinase activity non-receptor serine/threonine protein kinase
          Process(es)
          regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis glycogen metabolic process protein phosphorylation dopamine receptor signaling pathway nervous system development insulin receptor signaling pathway negative regulation of UDP-glucose catabolic process Wnt signaling pathway positive regulation of cAMP biosynthetic process negative regulation of TOR signaling cellular response to insulin stimulus cellular response to interleukin-3 IRE1-mediated unfolded protein response proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development positive regulation of adrenergic receptor signaling pathway negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway in absence of ligand chemical synaptic transmission, postsynaptic positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitophagy positive regulation of protein targeting to mitochondrion negative regulation of glycogen synthase activity, transferring glucose-1-phosphate negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity positive regulation of glycogen (starch) synthase activity re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion regulation of gene expression by genetic imprinting protein export from nucleus ER overload response axonogenesis myoblast fusion aging circadian rhythm organ morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation positive regulation of mitochondrial membrane potential negative regulation of neuron maturation response to activity cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation hippocampus development establishment of cell polarity negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process response to insulin regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation negative regulation of smooth muscle cell apoptotic process protein localization to microtubule intracellular signal transduction cellular response to hepatocyte growth factor stimulus cellular response to erythropoietin response to drug negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of neuron apoptotic process positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide superior temporal gyrus development cellular response to mechanical stimulus cellular response to iron(II) ion cellular response to lithium ion response to epinephrine regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin positive regulation of excitatory postsynaptic potential positive regulation of DNA biosynthetic process positive regulation of stem cell differentiation negative regulation of neuron migration signal transduction negative regulation of transferase activity cellular response to organic cyclic compound carbohydrate metabolic process negative regulation of signal transduction phosphorylation cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development cell proliferation regulation of neuron projection development negative regulation of neuron projection development myotube differentiation cell differentiation positive regulation of apoptotic process rhythmic process response to lithium ion regulation of cell cycle male gamete generation
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