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Invitrogen
TAF4 Monoclonal Antibody (20TA-1B12)
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FIGURE: 1 / 2
TAF4 Antibody (MA3-075) in ICC/IF
Immunofluorescent analysis of TAF4 in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a TAF4 mouse monoclonal antibody (Product # MA3-075) at a dilution of 1:4000 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilut... View More
Product Details
MA3-075
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
20TA-1B12
Immunogen
Recombinant TAF4 protein. Epitope is localized between aa 815 and 836.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-075 detects TATA-Binding Protein (TBP) Associated factor 4 from human and mouse samples.
MA3-075 has been successfully used in Western blot and immunofluorescence applications.
The MA3-075 immunogen is a recombinant TAF4 protein. Epitope is localized between aa 815 and 836.
Target Information
TAF II p105, also called TAF4B, is a celltype specific transcriptional co-activator that is a component of the TFIID complex. Expressed primarily in B cells and ovarian granulosa cells, TAF II p105 can interact with OCBA/POU2AF1 to activate B cell-specific octamerdependent transcription. Additionally, TAF II p105 plays an important role in co-activating the transcription factor NFkB and is essential for activation of anti-apoptotic genes such as TNFAIP3. TAF II p135, also known as TAF4, TAF2C, TAF2C1, TAF4A or TAFII130, is a 1,085 amino acid subunit of TFIID that accelerates transcriptional activation triggered by thyroid hormone (TR) or retinoic acid (RA). Localized to the nucleus, TAF II p135 contains one TAFH domain and is thought to bind to proteins that contain glutamine-rich domains, such as the transcription factor CREB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ41943; RNA polymerase II TBP-associated factor subunit C; TAF(II)130; TAF(II)135; TAF4 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135kDa; TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor, 135 kD; TAFII 130; TAFII 135; TATA box binding protein (TBP)-associated factor, RNA polymerase II, C1, 130kD; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; transcription initiation factor TFIID 135 kD subunit; Transcription initiation factor TFIID 135 kDa subunit; Transcription initiation factor TFIID subunit 4
Gene Aliases: 135kDa; AI450312; MRD73; TAF(II)130; TAF(II)135; TAF2C; TAF2C1; TAF4; TAF4A; TAFII-130; TAFII-135; TAFII130; TAFII135
UniProt ID: (Human) O00268
Entrez Gene ID: (Human) 6874, (Mouse) 228980
core promoter sequence-specific DNA binding
DNA binding
protein binding
obsolete general RNA polymerase II transcription factor activity
aryl hydrocarbon receptor binding
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription factor activity, sequence-specific DNA binding
general transcription factor
ovarian follicle development
regulation of DNA repair
transcription, DNA-templated
DNA-templated transcription, initiation
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
mRNA transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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