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Invitrogen
AID Monoclonal Antibody (1AID-2E11)
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FIGURE: 1 / 4
AID Antibody (MA3-081) in ICC/IF
Immunofluorescent analysis of AID (green) in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a AID mouse monoclonal antibody (Product # MA3-081) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a ... View More
Product Details
MA3-081
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1AID-2E11
Immunogen
Synthetic peptide coupled to Ovalbumin corresponding to aa 185 to 198 of mouse AID
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-081 detects AID from human, and mouse samples.
MA3-081 has been successfully used in Western blot, immunofluorescence, flow cytometry, and ELISA applications.
The MA3-081 immunogen is the synthetic peptide coupled to ovalalbumin, aa 185 to 198. MA3-081 detects Isoform 1 (~24kDa) and Isoform 2 (~22kDa) in human samples.
Target Information
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activation induced deaminase; Activation-induced cytidine deaminase; AID; AID isoform AIDins(IVS3); Cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; Single-stranded DNA cytosine deaminase
Gene Aliases: AICDA; AID; ARP2; CDA2; HEL-S-284; HIGM2
UniProt ID: (Human) Q9GZX7, (Mouse) Q9WVE0
Entrez Gene ID: (Human) 57379, (Mouse) 11628
mRNA processing
regulation of macromolecule biosynthetic process
somatic diversification of immunoglobulins
somatic hypermutation of immunoglobulin genes
cytidine to uridine editing
B cell differentiation
regulation of nuclear cell cycle DNA replication
defense response to bacterium
hypomethylation of CpG island
isotype switching
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
defense response to virus
DNA cytosine deamination
cellular response to lipopolysaccharide
cytidine deamination
DNA demethylation
negative regulation of methylation-dependent chromatin silencing
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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