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Invitrogen
NF-H Monoclonal Antibody (NAP4)
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FIGURE: 1 / 2
NF-H Antibody (MA3-16722) in IHC (P)
Immunohistochemistry analysis of NF-H in human cerebellar cortex section. Samples were incubated with NF-H monoclonal antibody (Product # MA3-16722). Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje ne... View More
Product Details
MA3-16722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Horse,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
NAP4
Immunogen
Native NF-H purified from bovine spinal cord.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.035% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody specifically recognizes the phosphorylated variant of NF-H subunit (~200-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; hSTEp; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC; unnamed protein product
Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH
UniProt ID: (Human) P12036, (Mouse) P19246
Entrez Gene ID: (Horse) 100064328, (Human) 4744, (Pig) 100156492, (Chicken) 417020, (Rat) 24587, (Mouse) 380684
structural constituent of cytoskeleton
protein binding
microtubule binding
kinesin binding
protein kinase binding
protein binding, bridging
dynein complex binding
structural constituent of postsynaptic intermediate filament cytoskeleton
structural molecule activity
toxic substance binding
nutrient reservoir activity
protein heterodimerization activity
microtubule cytoskeleton organization
axonogenesis
cell projection assembly
neurofilament bundle assembly
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
peripheral nervous system neuron axonogenesis
neurofilament cytoskeleton organization
axon development
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
regulation of organelle transport along microtubule
cellular response to leukemia inhibitory factor
ovarian follicle atresia
brain development
embryo development
spinal cord development
axon regeneration
neuron projection development
cellular response to oxidative stress
response to cocaine
cellular response to estradiol stimulus
response to sodium arsenite
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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