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Invitrogen
NONO Monoclonal Antibody (78-1 C)
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FIGURE: 1 / 14
NONO Antibody (MA3-2024) in IHC (P)
Immunohistochemistry was performed on cancer biopsies of deparaffinized human Bladder carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mon... View More
Product Details
MA3-2024
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
78-1 C
Immunogen
Synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-2024 detects the nmt55/p54nrb in human, rat and rabbit samples.
MA3-2024 has been successfully used in immunohistochemistry, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~55 kDa protein representing nmt55/p54nrb in MCF-7 cells, human breast tumor cells, rat uterine tissue, and rabbit liver extracts. In immunohistochemistry this antibody recognizes the nmt55/p54nrb in MCF-7 cells and human breast tumor cells. This monoclonal antibody has been referred to as
NMT-1.
The MA3-2024 immunogen is a synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.
Target Information
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; HGNC:7871; NMT-1; NMT55; non-POU domain-containing octamer (ATGCAAAT) binding protein; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing; non-POU-domain-containing, octamer-binding protein; NONO; NonO protein; p54(nrb); protein phosphatase 1, regulatory subunit 114; sequence is expressed in human Tera-2 clone 13 (embryonal carcinoma) cells. The sequence may contain mismatches (one strand sequenced only once). 97% identical in 320 bp overlap with human 54 kDA prot; ORF; unnamed protein product
Gene Aliases: MRXS34; NMT55; NONO; NRB54; P54; P54NRB; PPP1R114
UniProt ID: (Human) Q15233, (Rat) Q5FVM4
Entrez Gene ID: (Rabbit) 100346936, (Rabbit) 100348685, (Human) 4841, (Rat) 317259
activation of innate immune response
immune system process
DNA repair
DNA recombination
regulation of transcription, DNA-templated
mRNA processing
cellular response to DNA damage stimulus
circadian rhythm
RNA splicing
regulation of circadian rhythm
negative regulation of apoptotic process
innate immune response
negative regulation of transcription, DNA-templated
rhythmic process
cellular response to hypoxia
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
cellular response to angiotensin
transcription, DNA-templated
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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