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Invitrogen
p23 Monoclonal Antibody (JJ3)
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FIGURE: 1 / 31
p23 Antibody (MA3-414) in ICC/IF
Immunofluorescent analysis of p23 using Anti-p23 Monoclonal Antibody (JJ5) (Product # MA3-414) shows staining in Hela Cells. p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 (Product # MA3-414) at a dilution of 1:500 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat ... View More
Product Details
MA3-414
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Guinea pig,
Human,
Mouse,
Rabbit,
Rat
Published species
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
JJ3
Immunogen
Recombinant human p23 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples.
MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 19 or 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23.
The MA3-414 antigen is recombinant human p23.
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; Cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; p23; p23 cochaperone; Progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; ptges3 {ECO:0000250|UniProtKB:Q15185}; Sid 3177; telomerase binding protein, p23; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD; unnamed protein product
Gene Aliases: 5730442A20Rik; CPGES; P23; Ptges; PTGES3; RGD1561913; Sid3177; TEBP
UniProt ID: (Chicken) Q90955, (Human) Q15185, (Rat) P83868, (Mouse) Q9R0Q7
prostaglandin biosynthetic process
protein folding
telomere maintenance via telomerase
chaperone-mediated protein complex assembly
telomerase holoenzyme complex assembly
telomere maintenance
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
prostaglandin metabolic process
signal transduction
positive regulation of telomere maintenance via telomerase
prostanoid biosynthetic process
protein stabilization
glycogen biosynthetic process
RNA-dependent DNA biosynthetic process
cell proliferation
positive regulation of gene expression
sensory perception of pain
glucocorticoid receptor signaling pathway
skin development
lung saccule development
negative regulation of cell death
chaperone cofactor-dependent protein refolding
DNA biosynthetic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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