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Invitrogen

PMCA ATPase Monoclonal Antibody (5F10)

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20 Published Figures
100 References
View all (4) PMCA ATPase antibodies

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PMCA ATPase Monoclonal Antibody (5F10)

Product Details

MA3-914

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
View 68 publications 68 publications

Immunohistochemistry (IHC)

-
View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:500
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent
View 9 publications 9 publications

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
View 5 publications 5 publications

Neutralization (Neu)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Amphibian, Dog, Chicken, Cat, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

Published species

Amphibian, Bovine, Cat, Dog, Hamster, Human, Insect, Mouse, Non-human primate, Pig, Rabbit, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5F10

Immunogen

Purified human erythrocyte calcium ATPase.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061566

Product Specific Information

MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.

MA3-914 has been successfully used in Western blot, FACS, immunohistochemistry, immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~140 kDa protein representing PMCA ATPase and bands at 95kDa and 180 kDa which probably represent products of aggregation and/or natural proteolytic products of the pump from rat liver membrane preparations. Immunohistochemical staining of PMCA ATPase with MA3-914 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

MA3-914 can be used on both frozen and formalin-fixed paraffin-embedded tissues.

The MA3-914 antigen is purified human erythrocyte calcium ATPase. This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.

Target Information

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP-dependent plasma membrane calcium transporter; ATPase isoform 2 Na+K+ transporting beta polypeptide 2; ATPase isoform 2, Na+K+ transporting, beta polypeptide 2; ATPase, Ca++ transporting, plasma membrane 1; ATPase, Ca++ transporting, plasma membrane 2; ATPase, Ca++ transporting, plasma membrane 3; ATPase, Ca++ transporting, plasma membrane 4; Ca2+/Mg2+ ATPase; calcium pump; cilia and flagella associated protein 39; deaf waddler; Matrix-remodeling-associated protein 1; plasma membrane Ca(2+)-ATPase; plasma membrane Ca++-ATPase; plasma membrane Ca2+ pump 2; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium ATPase isoform 3; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 1; Plasma membrane calcium pump isoform 2; Plasma membrane calcium pump isoform 3; Plasma membrane calcium pump isoform 4; plasma membrane calcium-ATPase isoform 1 alternative splice variant k at splice site B and splice variant b at splice site C; plasma membrane calcium-ATPase isoform 2 alternative splice variant a at splice site C; plasma membrane calcium-ATPase isoform 2, splice variant i; plasma membrane calcium-ATPase isoform 3, splice variant a at alternative splice site C; plasma membrane calcium-ATPase isoform 4 alternative splice variant x at splice site A; plasma membrane calcium-ATPase isoform 4, splice variant b at alternative splice site C; Plasma membrane calcium-transporting ATPase 1; Plasma membrane calcium-transporting ATPase 2; Plasma membrane calcium-transporting ATPase 3; Plasma membrane calcium-transporting ATPase 4; PMCA1; PMCA1b; PMCA2; PMCA3; PMCA4; PMCA4a; PMCA4b; sarcolemmal calcium pump; unnamed protein product; wriggle

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Gene Aliases: 2810442I22Rik; 6430519O13Rik; ATP2B; ATP2B1; ATP2B2; ATP2B3; ATP2B4; CFAP39; CLA2; D6Abb2e; DFNA82; dfw; E130111D10Rik; Gena300; jog; MRD66; MXRA1; OPCA; PMCA; PMCA1; Pmca1a; Pmca1b; Pmca1c; PMCA1kb; PMCA2; PMCA2a; PMCA2i; PMCA3; PMCA3a; PMCA4; PMCA4b; PMCA4x; SCAX1; Tmy; wms; wri

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UniProt ID: (Rabbit) Q00804, (Human) P20020, (Chicken) Q98SH2, (Human) Q01814, (Human) Q16720, (Human) P23634, (Rat) P11505, (Mouse) G5E829, (Mouse) Q9R0K7, (Rat) P11506, (Rat) Q64568, (Mouse) Q6Q477, (Rat) Q64542

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Entrez Gene ID: (Rabbit) 100009543, (Human) 490, (Sheep) 101111268, (Chicken) 374244, (Dog) 403480, (Sheep) 101121046, (Human) 491, (Chicken) 415958, (Dog) 476535, (Sheep) 101101873, (Rabbit) 100356760, (Dog) 492238, (Human) 492, (Dog) 403731, (Human) 493, (Rabbit) 100353865, (Chicken) 419934, (Rat) 29598, (Mouse) 67972, (Mouse) 11941, (Rat) 24215, (Mouse) 320707, (Rat) 29599, (Mouse) 381290, (Rat) 29600

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Function(s)
nucleotide binding calcium-transporting ATPase activity protein binding calmodulin binding ATP binding calcium ion transmembrane transporter activity ATPase activity metal ion binding molecular function inhibitor activity calcium ion binding PDZ domain binding P-type calcium transporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration sodium channel regulator activity protein kinase binding protein phosphatase 2B binding nitric-oxide synthase inhibitor activity nitric-oxide synthase binding scaffold protein binding hydrolase activity protein C-terminus binding calcium-dependent ATPase activity acetylcholine receptor binding glutamate receptor binding primary active transporter
Process(es)
negative regulation of cytokine production regulation of vascular smooth muscle contraction ion transport calcium ion transport cellular calcium ion homeostasis regulation of blood pressure positive regulation of bone mineralization ion transmembrane transport regulation of cytosolic calcium ion concentration negative regulation of cytosolic calcium ion concentration positive regulation of calcium ion transport calcium ion transmembrane transport regulation of cellular response to insulin stimulus regulation of cardiac conduction calcium ion export from cell sensory perception of sound neuron differentiation regulation of postsynaptic cytosolic calcium ion concentration regulation of presynaptic cytosolic calcium ion concentration neural retina development regulation of transcription from RNA polymerase II promoter spermatogenesis negative regulation of gene expression urinary bladder smooth muscle contraction negative regulation of angiogenesis hippocampus development sperm motility nitric oxide-cGMP-mediated signaling pathway negative regulation of blood vessel endothelial cell migration negative regulation of nitric oxide biosynthetic process response to hydrostatic pressure negative regulation of calcineurin-NFAT signaling cascade cellular response to epinephrine stimulus negative regulation of adrenergic receptor signaling pathway calcium ion transmembrane import into cytosol calcium ion import across plasma membrane negative regulation of the force of heart contraction transport across blood-brain barrier negative regulation of arginine catabolic process calcium ion export regulation of sodium ion transmembrane transport negative regulation of cellular response to vascular endothelial growth factor stimulus regulation of cell cycle G1/S phase transition positive regulation of protein localization to plasma membrane negative regulation of cardiac muscle hypertrophy in response to stress negative regulation of citrulline biosynthetic process cellular response to acetylcholine brain development aging metabolic process response to cold cellular response to vitamin D cellular response to corticosterone stimulus transport cell morphogenesis organelle organization lactation locomotory behavior regulation of cell size cerebellum development cerebellar Purkinje cell layer morphogenesis cerebellar Purkinje cell differentiation cerebellar granule cell differentiation locomotion serotonin metabolic process inner ear morphogenesis otolith mineralization cGMP metabolic process regulation of synaptic plasticity inner ear development synapse organization neuromuscular process controlling balance detection of mechanical stimulus involved in sensory perception of sound auditory receptor cell stereocilium organization inner ear receptor cell differentiation cochlea development negative regulation of nitric oxide mediated signal transduction positive regulation of peptidyl-serine phosphorylation negative regulation of nitric-oxide synthase activity negative regulation of adrenergic receptor signaling pathway involved in heart process negative regulation of peptidyl-cysteine S-nitrosylation positive regulation of cAMP-dependent protein kinase activity
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