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Invitrogen
ATP1A1 Monoclonal Antibody (M7-PB-E9)
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FIGURE: 1 / 28
ATP1A1 Antibody (MA3-928) in ICC/IF
Immunofluorescent analysis of Sodiµm/Potassium ATPase alpha using Sodiµm/Potassium ATPase alpha Monoclonal Antibody (M7-PB-E9) (Product # MA3-928) shows staining in MCF-7 Cells. Sodiµm/Potassium ATPase alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Sodiµm/Potassium ATPase alpha (Product # MA3-928) at a dilution of 1:20 over night at 4 °C, washed wi... View More
Product Details
MA3-928
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Sheep,
Pig,
Rat
Published species
Dog,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M7-PB-E9
Immunogen
Purified alpha sodium/potassium ATPase from sheep kidney.
Conjugate
Form
Liquid
Concentration
1.87 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-928 detects sodium/potassium ATPase alpha from human, mouse, bovine, sheep, canine, chicken and porcine tissues. This antibody detects the alpha 3 isoform in rat only.
MA3-928 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, immunocytochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha sodium/potassium ATPase from MDCK cell extract. Immunohistochemical staining of alpha sodium/potassium ATPase in porcine heart with MA3-928 yields a pattern consistent with the plasma membrane localization.
The MA3-928 antigen is purified sheep kidney alpha sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 646 and 652 of the sheep kidney alpha sodium/potassium ATPase.
Target Information
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: (Na+ + K+)-ATPase; (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product
Gene Aliases: ATP1A1; Atpa-1; BC010319; Nkaa1b
UniProt ID: (Sheep) P04074, (Human) P05023, (Pig) P05024, (Bovine) Q08DA1, (Chicken) P09572, (Rat) P06685, (Mouse) Q8VDN2
Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Human) 476, (Pig) 397481, (Bovine) 282144, (Chicken) 396530, (Rat) 24211, (Mouse) 11928
sodium:potassium-exchanging ATPase activity
ATP binding
metal ion binding
protein binding
phosphatase activity
protein kinase binding
protein domain specific binding
ankyrin binding
potassium ion binding
sodium ion binding
ADP binding
phosphatidylinositol 3-kinase binding
chaperone binding
steroid hormone binding
ATPase binding
nucleotide binding
hydrolase activity
primary active transporter
regulation of sodium ion transport
sodium ion transport
establishment or maintenance of transmembrane electrochemical gradient
potassium ion transmembrane transport
regulation of the force of heart contraction
cellular sodium ion homeostasis
regulation of blood pressure
potassium ion import
ATP hydrolysis coupled proton transport
dephosphorylation
cellular potassium ion homeostasis
negative regulation of glucocorticoid biosynthetic process
ion transmembrane transport
sodium ion export from cell
response to drug
negative regulation of heart contraction
positive regulation of heart contraction
positive regulation of striated muscle contraction
relaxation of cardiac muscle
cardiac muscle contraction
membrane hyperpolarization
cellular response to mechanical stimulus
cellular response to steroid hormone stimulus
regulation of cardiac muscle cell contraction
membrane repolarization
membrane repolarization during cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
response to glycoside
regulation of cardiac conduction
potassium ion import across plasma membrane
transmembrane transport
potassium ion transport
energy coupled proton transmembrane transport, against electrochemical gradient
sodium ion transmembrane transport
transport
ion transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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