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Invitrogen

PARP1 Monoclonal Antibody (C-2-10)

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5 Published Figures
12 References
View all (61) PARP1 antibodies

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PARP1 Monoclonal Antibody (C-2-10)

Product Details

MA3-950

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100
-

Immunocytochemistry (ICC/IF)

Assay-dependent
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C-2-10

Immunogen

Purified calf thymus poly (ADP-ribose) polymerase.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325523

Product Specific Information

This antibody does not detect PARP from chicken tissues.

By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract.

This antibody recognizes an epitope found between amino acids 216-375 in the DNA-binding domain of PARP.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

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Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; I79_014850; pADPRT-1; PARP; PARP-1; PARP1; PARS; Poly-PARP; PPOL; sPARP-1

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UniProt ID: (Bovine) P18493, (Human) P09874, (Rat) P27008, (Chinese hamster) Q9R152

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Entrez Gene ID: (Bovine) 286764, (Human) 142, (Rat) 25591, (Chinese hamster) 100689463, (Mouse) 11545

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Function(s)
DNA binding zinc ion binding NAD binding protein ADP-ribosylase activity chromatin binding damaged DNA binding RNA binding catalytic activity NAD+ ADP-ribosyltransferase activity protein binding enzyme activator activity transferase activity transferase activity, transferring glycosyl groups nucleotidyltransferase activity enzyme binding protein kinase binding estrogen receptor binding nucleosome binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity histone deacetylase binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding R-SMAD binding NAD DNA ADP-ribosyltransferase activity transcription regulator activator activity NAD+-protein-serine ADP-ribosyltransferase activity NAD+-protein-aspartate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity NAD+-protein-tyrosine ADP-ribosyltransferase activity NAD+-protein-histidine ADP-ribosyltransferase activity NAD+-histone H2BS6 serine ADP-ribosyltransferase activity NAD+-histone H3S10 serine ADP-ribosyltransferase activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity transcription factor binding transferase activity, transferring pentosyl groups poly(A) RNA binding protein N-terminus binding DNA metabolism protein
Process(es)
double-strand break repair protein ADP-ribosylation cellular response to DNA damage stimulus positive regulation of cardiac muscle hypertrophy peptidyl-serine ADP-ribosylation peptidyl-glutamic acid poly-ADP-ribosylation DNA ADP-ribosylation protein poly-ADP-ribosylation protein auto-ADP-ribosylation positive regulation of double-strand break repair via homologous recombination ATP generation from poly-ADP-D-ribose negative regulation of transcription from RNA polymerase II promoter telomere maintenance double-strand break repair via homologous recombination immune system process DNA repair transcription from RNA polymerase II promoter apoptotic process mitochondrion organization transforming growth factor beta receptor signaling pathway response to gamma radiation carbohydrate biosynthetic process protein autoprocessing signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process positive regulation of DNA-templated transcription, elongation cellular response to insulin stimulus regulation of protein localization positive regulation of intracellular estrogen receptor signaling pathway negative regulation of transcription elongation from RNA polymerase II promoter cellular response to oxidative stress cellular response to UV protein modification process positive regulation of I-kappaB kinase/NF-kappaB signaling mitochondrial DNA repair innate immune response regulation of circadian sleep/wake cycle, non-REM sleep negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization positive regulation of mitochondrial depolarization positive regulation of SMAD protein import into nucleus positive regulation of necroptotic process protein localization to chromatin cellular response to zinc ion cellular response to transforming growth factor beta stimulus replication fork reversal DNA repair-dependent chromatin remodeling negative regulation of cGAS/STING signaling pathway transcription pausing by RNA polymerase II positive regulation of protein localization to nucleus cellular response to oxygen-containing compound regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway response to aldosterone negative regulation of adipose tissue development positive regulation of adipose tissue development negative regulation of telomere maintenance via telomere lengthening cellular response to beta-amyloid positive regulation of myofibroblast differentiation regulation of base-excision repair cellular response to nerve growth factor stimulus protein localization to site of double-strand break negative regulation of ATP biosynthetic process DNA metabolic process base-excision repair transcription, DNA-templated regulation of growth rate DNA damage response, detection of DNA damage cellular response to superoxide positive regulation of transcription regulatory region DNA binding regulation of transcription, DNA-templated behavioral response to cocaine regulation of single strand break repair
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