Immunofluorescent analysis of Actin pan (green) showing staining in the cytoplasm of T-47D cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin pan monoclonal antibody (Product # MA5-11869) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Actin Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image Actin Monoclonal Antibody (ACTN05 (C4))

Product Details

MA5-11869

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 369 publications 369 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Immunocytochemistry (ICC/IF)

1:10-1:200

View 6 publications 6 publications

Immunoprecipitation (IP)

2 µg

View 6 publications 6 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Human, Mouse, Pig, Protozoa, Rabbit, Rat

Published species

C. elegans, Chicken, Chordate, Dog, Ferret, Fish, Fruit fly, Hamster, Human, Insect, Invertebrate, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Xenopus, Yeast, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ACTN05 (C4)

Immunogen

Chicken gizzard actin

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11004139

Product Specific Information

MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.

Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.

MA5-11869 is a suggested replacement to Product # 82353, clone # C4.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: a-actin; ACT2; ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin 2; actin 7; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; AtACT7; Atcb; B-actin; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; DEFORMED ROOT HAIRS 1; DER1; ENHANCER OF LRX1 2; ENL2; epididymis luminal protein 176; FIZ2; FRIZZY AND KINKED SHOOTS 2; gamma 1 propeptide; gamma non-muscle actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; I(2)-actin; LIGHT STRESS-REGULATED 2; LSR2; MYH9.2; MYH9_2; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; Bact; beta-actin; BKRNS; BNS; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; CSMH; DDS1; DFNA20; DFNA26; E430023M04Rik; E51; GAMMA-ACTIN; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMDYS; SMGA; THC8; VSCM; VSCM1

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Entrez Gene ID: (Human) 58, (Chicken) 421534, (Bovine) 281592, (Rabbit) 100009506, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Chicken) 396526, (Dog) 403580, (Dog) 478250, (Bovine) 533219, (Chicken) 423298, (Human) 70, (Rabbit) 100340339, (Pig) 100152267, (Chicken) 415296, (Dog) 475923, (Human) 71, (Bovine) 404122, (Pig) 397653, (Bovine) 281595, (Chicken) 396084, (Pig) 100520667, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365

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Function(s)

nucleotide binding structural constituent of cytoskeleton protein binding ATP binding hydrolase activity myosin binding ADP binding protein kinase binding kinesin binding Tat protein binding identical protein binding nitric-oxide synthase binding ATPase activity nitric-oxide synthase regulator activity nucleosomal DNA binding tau protein binding structural constituent of postsynaptic actin cytoskeleton transporter regulator activity microfilament motor activity profilin binding ubiquitin protein ligase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding molecular_function actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

Performance Guarantee

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Actin Monoclonal Antibody (ACTN05 (C4))

Actin Antibody (MA5-11869) in ICC/IF

Product Details

MA5-11869

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunomicroscopy (IM)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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