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Invitrogen
Actin Monoclonal Antibody (ACTN05 (C4))
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FIGURE: 1 / 77
Actin Antibody (MA5-11869) in ICC/IF
Immunofluorescent analysis of Actin pan (green) showing staining in the cytoplasm of T-47D cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin pan monoclonal antibody (Product # MA5-11869) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-11869
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Human,
Mouse,
Pig,
Protozoa,
Rabbit,
Rat
Published species
C. elegans,
Chicken,
Chordate,
Dog,
Ferret,
Fish,
Fruit fly,
Hamster,
Human,
Insect,
Invertebrate,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Tag,
Xenopus,
Yeast,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
ACTN05 (C4)
Immunogen
Chicken gizzard actin
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.
MA5-11869 is a suggested replacement to Product # 82353, clone # C4.
Target Information
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a-actin; A-X actin; A-X actin-like protein; ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; actin, beta, cytoplasmic; actin, cytoplasmic 2; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin-like protein; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; Alpha-actin-1; alpha-actin-2; alpha-actin-3; alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; B-actin; Bact; actin; beta actin; beta cytoskeletal actin; beta-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic protein; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; epididymis luminal protein 176; gamma 1 propeptide; gamma non-muscle actin; gamma-2-actin; gamma-actin; gamma-enteric smooth muscle actin; GIG46; I(2)-actin; melanoma X-actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; skeletal alpha actin; skeletal muscle alpha-actin; smooth muscle alpha-actin; smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin
Gene Aliases: 0610041G09Rik; a-SMA; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA3; ACTC; Actc-1; ACTE; ACTG; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; Actx; alphaSMA; ASD5; ASMA; Bact; beta-actin; BKRNS; BNS; BRWS1; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; CSMH; DDS1; DFNA20; DFNA26; E430023M04Rik; E51; GAMMA-ACTIN; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMAalpha; SMalphaA; SMDYS; SMGA; THC8; VSCM; VSCM1
UniProt ID: (Human) P68133, (Chicken) P68139, (Bovine) P68138, (Rabbit) P68135, (Pig) P68137, (Rabbit) P62740, (Chicken) P08023, (Bovine) P62739, (Human) P62736, (Rabbit) P29751, (Bovine) P60712, (Pig) Q6QAQ1, (Human) P60709, (Chicken) P60706, (Dog) O18840, (Bovine) Q3ZC07, (Chicken) P68034, (Human) P68032, (Chicken) P53478, (Human) P63261, (Bovine) P63258, (Bovine) Q5E9B5, (Chicken) P63270, (Human) P63267, (Mouse) P68134, (Rat) P68136, (Rat) P62738, (Mouse) P62737, (Rat) P60711, (Mouse) P60710, (Rat) P68035, (Mouse) P68033, (Mouse) P63260, (Rat) P63259, (Rat) P63269, (Mouse) P63268
Entrez Gene ID: (Human) 58, (Chicken) 421534, (Bovine) 281592, (Rabbit) 100009506, (Pig) 100154254, (Dog) 488984, (Pig) 733615, (Dog) 477587, (Rabbit) 100009271, (Chicken) 423787, (Bovine) 515610, (Human) 59, (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Chicken) 396526, (Dog) 403580, (Dog) 478250, (Bovine) 533219, (Chicken) 423298, (Human) 70, (Rabbit) 100340339, (Pig) 100152267, (Chicken) 415296, (Dog) 475923, (Human) 71, (Bovine) 404122, (Pig) 397653, (Bovine) 281595, (Chicken) 396084, (Pig) 100520667, (Human) 72, (Rabbit) 100350318, (Dog) 475792, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Rat) 29275, (Mouse) 11464, (Mouse) 11465, (Rat) 287876, (Rat) 25365, (Mouse) 11468
structural constituent of cytoskeleton
protein binding
ATP binding
ATPase activity
myosin binding
ADP binding
protein kinase binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
nitric-oxide synthase regulator activity
nucleosomal DNA binding
tau protein binding
structural constituent of postsynaptic actin cytoskeleton
transporter regulator activity
microfilament motor activity
profilin binding
ubiquitin protein ligase binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
muscle contraction
positive regulation of gene expression
skeletal muscle thin filament assembly
skeletal muscle fiber development
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
cytoskeleton organization
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
positive regulation of ERK1 and ERK2 cascade
cellular response to transforming growth factor beta stimulus
juxtaglomerular apparatus development
positive regulation of hepatic stellate cell activation
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
microtubule nucleation
axonogenesis
positive regulation of cell proliferation
substantia nigra development
regulation of mitotic metaphase/anaphase transition
maintenance of blood-brain barrier
regulation of apoptotic process
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cell motility
positive regulation of norepinephrine uptake
regulation of cell cycle
regulation of G0 to G1 transition
platelet aggregation
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
positive regulation of stem cell population maintenance
positive regulation of double-strand break repair via homologous recombination
regulation of G1/S transition of mitotic cell cycle
regulation of double-strand break repair
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
actin filament organization
actin-myosin filament sliding
heart contraction
actin filament-based movement
actomyosin structure organization
cardiac myofibril assembly
cardiac muscle tissue morphogenesis
angiogenesis
positive regulation of cell migration
regulation of stress fiber assembly
regulation of focal adhesion assembly
positive regulation of wound healing
tight junction assembly
protein localization to bicellular tight junction
response to ethanol
cellular response to interleukin-6
cellular response to acetaldehyde
response to mechanical stimulus
cellular response to potassium ion
skeletal muscle fiber adaptation
response to steroid hormone
circadian rhythm
dynein-driven meiotic oscillatory nuclear movement
response to immobilization stress
retina development in camera-type eye
cellular response to electrical stimulus
response to xenobiotic stimulus
negative regulation of apoptotic process
cardiac muscle contraction
cytoplasmic actin-based contraction involved in cell motility
sarcomere organization
cellular response to interferon-gamma
response to calcium ion
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