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Invitrogen
Mouse anti-Human Kappa Light Chain Secondary Antibody, Biotin
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FIGURE: 1 / 7
Human Kappa Light Chain Secondary Antibody (MA5-12114) in WB
Western blot analysis of immunoglobulin (Ig) kappa light chain was performed by loading the indicated amounts of purified human IgG/kappa and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temp... View More
Product Details
MA5-12114
Applications
Tested Dilution
Western Blot (WB)
1:400-1:1,600
Immunohistochemistry (Paraffin) (IHC (P))
1:400-1:800
Flow Cytometry (Flow)
1 µg/mL
ELISA (ELISA)
1 µg/mL
Immunoprecipitation (IP)
2 µg
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
L1C1
Immunogen
B lymphoma cells
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
Kappa light chain
Antibody Form
Whole Antibody
Product Specific Information
By Western blot, MA5-12114 detects Human Ig kappa light chain chain at ~25kD in human samples. At high concentrations, the antibody will also detect heavy chain.
By ELISA and WB, MA5-12114 does not react with human Ig lambda light chain.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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