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Invitrogen
Pds1 Monoclonal Antibody (DCS-280)
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Product Details
MA5-12252
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
DCS-280
Immunogen
Human recombinant full length Pds1 protein
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
IHC staining of formalin-fixed tissues requires boiling in 10mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling at room temperature for 20 minutes. HeLa or tonsil tissues can be used as positive controls.
Target Information
Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle check point pathways. Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1, cystein protease that causes cleavage of the cohesin Scc1 that binds the sister chromatids together. Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle. Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is a prerequisite for anaphase entry. Pds1 is targeted for degradation by ubiquitination mediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitin ligase. In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: somatomedin A
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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