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Invitrogen
CA125 Monoclonal Antibody (M11)
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FIGURE: 1 / 2
CA125 Antibody (MA5-12425) in ICC/IF
Immunofluorescence analysis of CA 125 was performed using 70% confluent log phase SKOV3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA 125 (M11) Mouse Monoclonal Antibody (Product # MA5-12425) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
Product Details
MA5-12425
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
M11
Immunogen
CA 125 antigen derived from ascites fluid
Conjugate
Form
Liquid
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-12425 targets CA 125 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12425 immunogen is cA 125 antigen derived from ascites fluid.
Target Information
CA 125 determinant is present on a high molecular weight, mucin like glycoprotein of high molecular weight. CA 125 has been found on frozen sections of amnion and derivatives of coelomic and mullerian epithelium, including pleura, pericardium and peritoneum. In adult tissues, epithelial cells of fallopian tube, endometrium and endocervix; pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland and mammary gland. It is also found in mesothelial cell lining of pleura, pericardium and peritoneum. It is found in ovarian tumors of serous, endometrioid or clear cell types and adenocarcinomas of mullerian type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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