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Invitrogen
Ku80 Monoclonal Antibody (111)
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FIGURE: 1 / 22
Ku80 Antibody (MA5-12933) in ICC/IF
Immunofluorescence analysis of Ku80 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ku80 Monoclonal Antibody (111) (Product # MA5-12933) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175... View More
Product Details
MA5-12933
Applications
Tested Dilution
Publications
Product Specifications
Published species
Fruit fly,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
111
Immunogen
Human B cell nuclei from plasmacytoid 2p68 cells
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12933 targets Ku (p80) in FACS, ICC/IF, IP, and WB applications and shows reactivity with Human, Mouse and Non-human primate samples. This antibody does not cross-react with mouse in immunofluorescent applications, but does react with mouse in FACS applications.
The MA5-12933 immunogen is human B cell nuclei from plasmacytoid 2p68 cells.
Target Information
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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